Abstract

Objective: To evaluate the antibacterial activity of flavanoids, Orientin and Vicenin, obtained from leaves of Ocimum sanctum, have also been compared by their combine sample. Methods: Aqueous extract of fresh leaves of Ocimum sanctum was assessed for the isolation and purification of different flavanoids. The antibacterial activity of Orientin, Vicenin and Combine sample of both these flavanoids was evaluated according to well diffusion method against some bacteria causing Urinary Tract Infection (UTI) in human. Results: The result indicated that the combined sample or synergistic activity of both individual flavanoids showed positive result against Escherichia coli, Proteus, Staphylococcus aureus, Staphylococcus cohni and Klebsialla pneumonia with zone of inhibition 20.12, 20.75, 20.95, 19.55 and 20.1 mm at concentration of 400 mg/ml respectively. But the individual flavanoids showed the positive result against only limited microorganism. Conclusions: The finding of the present study provides the evidence that this flavanoid sample is used as an antibacterial agent. This is also beneficial to use this combine sample of different flavanoids of Ocimum sanctum for medication and other purposes.

Authors and Affiliations