In vitro Antioxidant, Antimicrobial and in vivo Peripheral Analgesic Activities of Methanol and Petroleum Ether Extracts of Whole Plant of Uraria lagopoides DC
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 8, Issue 5
Abstract
Aims: To study and evaluate in vitro antioxidant, antimicrobial and in vivo peripheral analgesic activities of both methanol and petroleum ether extracts of whole plant of Uraria lagopoides DC (Family-Fabaceae). Study Design: Preliminary phytochemical screening, evaluation of antioxidant, antimicrobial and peripheral analgesic activities. Place and Duration of Study: Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342 and Department of Pharmacy, Primeasia University, Dhaka-1213. The studies were carried out during February- September 2013. Methodology: Phytochemical constituents were identified by qualitative analysis. In vitro antioxidant activity of the extracts were studied using DPPH radical scavenging assay, total phenol, total flavonoid content and total antioxidant capacity determination assays. Antimicrobial activity was investigated by disc diffusion technique and acetic acid induced writhing test was used for the evaluation of in vivo peripheral analgesic activity of the whole plant extracts. Results: Phytochemical screening for both extracts showed positive results for carbohydrates, flavonoids and glycosides while only methanol extract showed positive results for alkaloids, saponins, steroids and tannins. Methanol extract of the plant showed higher total phenolic content and as well as higher DPPH free radical scavenging activity than petroleum ether extract. On the other hand, petroleum ether extract showed higher total flavonoid content and total antioxidant capacity than methanol extract. In disc diffusion technique among six bacterial species, methanol extract showed concentration dependent activity against one Gram positive (Staphylococcus aureus) and three Gram negative bacteria (Escherichia coli, Salmonella typhi and Klebsiella pneumoniae) while petroleum ether extract showed concentration dependent activity against only one Gram negative bacteria (Salmonella typhi). In acetic acid induced writhing test both methanol and petroleum ether extracts of 250 mg/kg and 500 mg/kg (per oral) both doses showed significant (P < 0.001) analgesic activity. Conclusion: The results demonstrate that methanol and petroleum ether extracts of whole plant of U. lagopoides can be used as a potential source of antioxidant, antimicrobial and analgesic agent.
Authors and Affiliations
Md. Monir Hossain, Md. Nur Alam, Nizam Uddin, Mohammad Burhan Uddin, A. Y. Sk. Ferozuddin A. Chowdhury
Keywords
Related Articles
- EP ID EP344266
- DOI 10.9734/BJPR/2015/8884
- Views 56
- Downloads 0
GCMS Analysis of Leaf and Salt Stress Callus of Eggplant (Solanum melongena L.)
In the present study, the leaf explant cultured on a MS medium supplemented with BAP (4.44 μM) + NAA (0.98 μM) had the ability to induce more amount of green and friable callus. The 40 days old best grown callus was sub...
Assessment of Mutagenic Effects of Sulfadoxine-Pyrimethamine (SP) on Animal Model
Introduction: The use of medicines in pregnancy has always been a source of concern because of adverse effects on the foetus. Aim: The study therefore investigated the mutagenic potential of sulfadoxine-pyrimethamine (SP...
Use of Xanthan Gum in the Formulation of Diethylcarbamazine for Targeted Drug Delivery
Matrix tablets were prepared using xanthan gum (XG) and Diethylcarbamazine was used as model drug. The controlled release of the drug by the polymer matrices in the upper gastrointestinal tract (GIT) was assessed through...
Assessment of Organ Damage Markers in Acetaminophen-induced Hepatotoxicity in Rats Pre-treated with Quail Eggs
This study investigated the response of organ damage markers to quail egg pre-treatment on acetaminophen–induced hepatotoxicity in rats. Thirty (30) albino wistar rats assigned to 5 groups of 6 rats each were used for th...
Biological Activities of Nitro Steroids
The present review describes the biological activities of synthetic nitro steroids. More than fifty biologically active nitro steroids showed an analeptic, antineoplastic, anesthetic, neuroprotector, immunosuppressant, d...