IN VITRO ANTIOXIDANT POTENTIAL, FREE RADICAL SCAVENGING AND CYTOTOXIC ACTIVITY OF SIMAROUBA GLUACA LEAVES.
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 2
Abstract
Objective: To investigate the total phenolic, flavonoids, tannin contents of Simarouba glauca leaves and evaluate the antioxidant potential and cytotoxic activity in different human cell lines.Methods: The methanol, ethanol and water extracts of S. glauca leaves were examined for total phenolics, flavonoid and tannin content. Its antioxidant properties using FRAP, Phosphomolybdenum, Ferric ferrozine assays and free radical scavenging using DPPH were determined. Further, the reducing power and iron chelating effect of the extract using spectrophotometric assays were estimated. Finally, the cytotoxic activity against few human cancer cell lines were also examined using MTT assay.Results: The phytochemical investigations revealed that S. glauca leaves has only 0.14 to 0.18% of flavonoids, 250-400µg/mg phenolics and 67-200µg/mg tannin content in various solvent extracts. The extracts exhibited good reducing power with similar EC50 values aproximately in the range of 57-61µg /ml. Further, the leaf extracts showed iron chelation effect which was more pronounced in aqueous extract with IC50 value of 332µg/ml and exhibited very strong DPPH radical scavenging activity with IC50 vlaues ranging from 9-13µg/ml in various extracts. The methanolic extracts showed good antioxidant potential using FRAP and phopshomolybdenum methods, whereas the aqueous extract exihibited more pronounced antioxidant activty using sensitive ferric ferrozine assay with 580µg AAE/mg extract. The methanolic extract showed strong cytotoxic effect on SCC9 cancer cell line and less potent on HCT116 cancer cells.Conclusion: The findings suggests that Simarouba glauca leaf extract contain bioactive molecules which exhibit antioxidant activity that could be synergisticaly influencing the cytotoxic activity in selected cancer cell lines.Â
Authors and Affiliations
T. G. Umesh
Keywords
Related Articles
- EP ID EP579106
- DOI -
- Views 84
- Downloads 0
PLANT BASED QUORUM SENSING INHIBITORS OF PSEUDOMONAS AERUGINOSA
Phytochemical research has gained a lot of momentum in the medical field for the discovery of new, safe, and effective remedies. In the context of antimicrobial research, various plant sources have been discovered with t...
VALIDATED STABILITY-INDICATING RP-HPLC ASSAY METHOD FOR AZATHIOPRINE IN PHARMACEUTICAL DOSAGE FORM ACCORDING TO ICH GUIDELINES
Objective: A simple, sensitive, precise and accurate stability-indicating HPLC method has been developed and validated for determination of Azathioprine in bulk drug and in pharmaceutical dosage form in the presence of d...
EFFICACY OF MICRODOSES POTENTIZED HOMEOPATHIC DRUG, ARSENICUM ALBUM INDUCED BY ARSENIC TRIOXIDE IN ZEBRAFISH (DANIO RERIO)-A FTIR STUDY IN GILLS
Objective: Arsenic, one of the major natural as well as anthropogenic pollutant produces biochemical changes in the organs of animals due to its potential toxicity. Arsenic is toxic to fish and may be taken through gills...
PROTECTIVE EFFECTS OF ZINGIBER OFFICINALE AGAINST CARBON TETRACHLORIDE INDUCED LIVER FIBROSIS
Objective: Liver plays a pivotal role in regulating various physiological processes in the body such as metabolism, secretion, and storage. It has a great capacity to detoxify toxic substances and synthesize useful princ...
METHOD DEVELOPMENT AND VALIDATION OF NORETHINDRONE ACE-TATE ASSAY AND ITS RELATED IMPURITIES IN API AND PHARMACEUTICAL FORMULATION WITH ORTOGONAL DETECTOR TECHNQUES
Objective: To develop and validate a sensitive and stability indicating gradient reverse phase ultra-high performance(UHPLC-PDA) liquid chromatography with photodiode array(PDA) and mass spectroscopy (MS) of Norethindron...