In vitro Antisalmonellal and Antioxidant Properties of Mangifera indica L. Stem Bark Crude Extracts and Fractions
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 5, Issue 1
Abstract
Aims: Typhoid fever is still a major public health problem in developing countries, where it remains endemic. In view of searching for new antityphoid substances, the antisalmonellal and antioxidant properties of extracts and fractions of Mangifera indica L. stem bark were assessed. Study Design: Extraction, fractionation, antibacterial and antioxidant evaluation, phytochemical screening. Place and Duration of Study: Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon, between October 2013 and May 2014. Methodology: Different fractions, i.e. hexane, ethyl acetate and residual fractions, were obtained from methanol/methylene chloride (MeOH/CH2Cl2) (1:1) extract of Mangifera indica L. stem bark by partitioning. Various aqueous extracts were also prepared by decoction, infusion and maceration. All these extracts and fractions were subjected to antibacterial evaluation against Salmonella Typhi, Salmonella Paratyphi A, Salmonella Paratyphi B and Salmonella Typhimurium isolates, and a strain of Salmonella Typhi ATCC 6539, using the microdilution method. The evaluation of antioxidant properties, quantitative determination of total phenols and flavonoids, and phytochemical screening of these extracts and fractions were also performed. Results: The antimicrobial results showed that Mangifera indica L. extracts and fractions exhibited minimum inhibitory concentrations (MICs) ranging from 64 to 1024 µg/mL. The extracts and fractions exhibited antioxidant properties, e.g. IC50 = 10.00 µg/mL for the MeOH/CH2Cl2 extract and fractions, and 11.99 µg/mL for the infused extract. Significant amounts of total phenols and flavonoids were detected. Phytochemical screening showed the presence of alkaloids, flavonoids, tannins, anthraquinones, triterpenes and steroids in all the extracts and fractions. Conclusion: In the light of the foregoing, it was obvious that Mangifera indica L. stem bark contain antisalmonellal and antioxidant principles which could be developed for the treatment of enteric fevers (typhoid and paratyphoid fevers) and the management of oxidative stress induced by the infection. These findings support the claim of the local community about the use of this plant for the treatment of typhoid fever.
Authors and Affiliations
Landric Romeo Noghogne, Donatien Gatsing, Fotso . , Norbert Kodjio, Jean Baptiste Sokoudjou, Jules Roger Kuiate
Keywords
Related Articles
- EP ID EP344357
- DOI 10.9734/BJPR/2015/13390
- Views 69
- Downloads 0
Identification of Phenolic Compounds in Hibiscus sabdariffa Polyphenolic Rich Extract (HPE) by Chromatography Techniques
The study was designed to determine the phenolic constituents of Hibiscus sabdariffa polyphenolic rich extract (HPE), (a group of phenolic compounds occurring in the dried calyx of the red variety of Hibiscus sabdariffa)...
Glyphaea brevis (Spreng.) Monach.: A Review of the Ethno-medical, Phytochemical and Pharmacological Investigations
Ethnopharmacological Relevance: Glyphaea brevis (spreng) Monach. belongs to the family Tiliaceae. Traditionally it is used in Africa and South America to treat various disease conditions of man including fevers, gonorrhe...
mAb Higher Order Structure Analysis with Protein Conformational Array ELISA
The clinical and biological properties of protein-based therapeutics, or biologics, are closely related to their Higher Order Structures (HOS) which in turn can be altered by many physical and chemical conditions. A nove...
In-vitro Antimicrobial Potential of Cassia Genus – An Overview
Antibiotics are one of the most important weapons in fighting bacterial infections and have greatly benefited the health-related quality of human life since their introduction. Infectious diseases caused by microorganism...
Effect of Vitamin D Supplementation in Patients of Moderate Asthma Undergoing Treatment with Inhaled Corticosteroids
Background: Vitamin D is an essential nutrient with significant immunomodulatory effects. There is potential for a protective role of vitamin D against etiopathogenesis of asthma and morbidity. This study evaluated the e...