In vitro biofilm formation in ESBL producing Escherichia coli isolates from cage birds
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2016, Vol 9, Issue 11
Abstract
Objective: To determine biofilm and hydrophobicity formation ratios in extended spectrum beta lactamases (ESBL) synthesizing Escherichia coli isolates which were isolated from feces samples of 150 cage bird species randomly taken from pet shops in Hatay province, Turkey. Methods: In vitro biofilm production of 4 ESBL positive isolates were performed by Congo Red Agar (CRA), Standard Tube (ST) and Microtitre Plate (MP) methods while their hydrophobicity were examined by bacterial adhesion to hydrocarbon (BATH) test. Results: In the examined isolates, while biofilm production was found to be negative by CRA method, highest biofilm producing strain, among 4 bacteria was determined to be A42 by ST and MP methods. The Scanning Electron Microscopy (SEM) also displayed these confirmed findings. The hydrophobicity values of strains were determined to be between 22.45% and 26.42%. Conclusions: As a result, biofilm formation in cage bird feces originated ESBL positive Escherichia coli isolates was performed for the first time in Turkey. In order to present the relation between pathogenicity and biofilm production in animal originated ESBL positive isolates, further studies are required.
Authors and Affiliations
Ebru Sebnem Yılmaz
Keywords
Related Articles
- EP ID EP281732
- DOI 10.1016/j.apjtm.2016.10.003
- Views 105
- Downloads 0
Microsporidiosis in Iran: A systematic review and meta-analysis
Mahmoud Mahami-Oskouei1,2
Inhibitory activities of extracts of Rumex dentatus, Commelina benghalensis, Ajuga bracteosa, Ziziphus mauritiana as well as their compounds of gallic acid and emodin against dengue virus
Objective: To investigate the inhibitory effects against dengue virus serotype 2 (DENV-2) by five different fractions (extracted by methanol, ethanol, benzene, chloroform and n-hexane) of Rumex dentatus, Commelina bengha...
Frequency of typhoon occurrence accounts for the Poisson distribution of human leptospirosis cases across the different geographic regions in the Philippines
Objective: To study the distribution of human leptospirosis cases across the different geographic regions in the Philippines in 2015-2017 and to determine the relationship between the frequency of typhoon occurrence and...
Phytochemical analysis and biological activities of Hertia cheirifolia L. roots extracts
Objective: To test the antioxidant, antimicrobial and a-glucosidase inhibitory activities of the roots extracts from Hertia cheirifolia (H. cheirifolia) L. Methods: Total phenolics and total flavonoids content of the dif...
Genetic diversity and phylogenetic analysis of EG95 sequences of Echinococcus granulosus: Implications for EG95 vaccine application
Objective: To analyse the genetic variability of EG95 sequences and provide guidance for EG95 vaccine application against Echinococcus granulosus (E. granulosus). Methods: We analysed EG95 polymorphism by collecting tota...