IN VITRO CLONAL PROPAGATION OF DIPTERACANTHUS PROSTRATUS (POIR.) NEES. – A VALUABLE MEDICINAL PLANT
Journal Title: Innoriginal: International Journal Of Sciences - Year 2015, Vol 2, Issue 6
Abstract
High frequency in vitro plantlet regeneration protocol for Dipteracanthus prostratus has been developed in this study. Nodal shoot segments were used as explants to establish the cultures. The explants were sterilized using 0.1% HgCl2 solution for 4-5 min. The Murashige and Skoog (MS) medium augmented with 1.0 mg l-1 6-benzylaminopurine (BAP) was reported suitable for bud break from the nodal meristems of the explants. About 4.6 shoots with 3.7 cm length were induced per explant on this medium combination. The shoots were multiplied (31.7 shoots with 5.6 cm length) on MS medium supplemented with 0.5 mg l-1 each of BAP and Kinetin (Kin). The healthy and sturdy shoots were rooted in vitro on half-strength MS medium fortified with 1.5 mg l−1 indole-3-butyric acid (IBA). The rooted plantlets were hardened in the greenhouse using soilrite first in the bottles for 4 week and then in the paper cups for 2 weeks. The hardened plants were transferred to the earthen pots and finally shifted to the natural fields. About 96% plantlets were survived in the field conditions. The developed protocol could be used for mass propagation of this important medicinal plant. The superior planting material can be supplied to the farmers and drug manufactures.
Authors and Affiliations
Mahipal S Shekhawat, M Manokari, C. P Ravindran
Keywords
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