In vitro conservation of Ipomoea batatas (L.) Lam cultivars by minimum growth with the use of mannitol
Journal Title: Biotecnología Vegetal - Year 2019, Vol 19, Issue 1
Abstract
In vitro conservation of Ipomoea batatas (L.) Lam allows the exchange of germplasm and its availability for breeding programs. The objective of this work was to determine the effect of mannitol and abscisic acid in the conservation through minimum growth of I. batatas cultivars from INIVIT Germplasm Bank. Treatments included abscisic acid (ABA) (5 and 10 mg l-1) and mannitol (1.0, 1.5 and 2.0%) in a basal MS culture medium, the combination of ABA (10 mg l-1) and mannitol (1.0 , 1.5 and 2.0%). As controls were used the MS basal culture medium and MS with sorbitol (1.0%) and glucose (1.0%). In the cultivars 'Cautillo' and 'INIVIT BS 16-2006' the best results of survival, growth decrease and green but small leaves were obtained in the treatments that contained the basal culture medium with mannitol (10, 15 and 20%) . Growth of the explants was not achieved in culture media with abscisic acid. The MS culture medium with 2 mg l-1 of thiamine, 100 mg l-1 of myo-inositol and 1.0% of mannitol, allows the in vitro conservation of sweet potato cultivars between six and eight months.
Authors and Affiliations
Aymé Rayas Cabrera, Jorge López Torres, Víctor R Medero Vega, Milagros Basail Pérez, Arletys Santos Pino, Yenisey Gutiérrez Sánchez
Keywords
Related Articles
- EP ID EP682887
- DOI -
- Views 241
- Downloads 0
Effect of the planting material on the incidence of dry rot in Colocasia esculenta (L.) Schott and Xanthosoma spp.
The cocoyam (Xanthosoma spp. and Colocasia esculenta) is a monocot plant with vegetative propagation. This work was developed with the aim of determining the effect of the planting material type on the incidence of dry...
Effect of pruning in biomass production and steviol glycosides contents of Stevia rebaudiana Bertoni var. Morita II
For the production of dry leaf of Stevia rebaudiana Bertoni under the Cuban climatic conditions, it is necessary to identify the agrotechnical variables with the greatest impact on its yield. Therefore, the objective of...
Protocol for the Kalanchoe blossfeldiana Poelln. macropropagation
Within the genus Kalanchoe Adans, the K. blossfeldiana Poelln species is the most popular for the beauty of its flowering. This constitutes one of the best known ornamental pot plants in the world, occupying one of the h...
Exploring biocontrol efficacy of Trichoderma spp. against Fusarium sacchari, the causal agent of sugarcane wilt
Wilt caused by Fusarium sacchari, is one of the major diseases of sugarcane (Saccharum spp.) causing considerable economic loss. Trichoderma spp. are important biocontrol agents which have been exploited effectively for...
Potential of Trichoderma species as biofertilizer and biological control on Oryza sativa L. cultivation
Rice (Oryza sativa L.) is one of the main staples around the world, especially in Asia, the most consuming region. Rice cultivation currently faces many environmental problems caused by the contribution of chemical ferti...