In vitro crop physiology
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 1
Abstract
The culture procedures described up to the eighties, did not made any mention to the environmental control of in vitro plant development. However, growth rate, development and many of the physiologic-morphologic features of the in vitro grown plants are influenced by the culture vessel. The increasing knowledge about the environmental control of culture vessels under sterile conditions, is helping to change micorpropagation procedures. The in vitro environment with lower rate ventilation, brings about low flow rates of matter and energy, with minimum variations of temperature, high relative humidity and large daily changes of the concentration of CO2 inside the culture vessel. The type of culture vessel (size, shape, fabric and closing system) can influence the evolution of the atmosphere along the time of culture. Although submitted to different stresses factors plant can be grown in vitro, but plants can be faulty in their anatomy, morphology and physiology. As a consequence, these plants shown a phenotype unable to survive to ex vitro conditions. Different strategies can be used to control the atmosphere along the different phases of micropropagation, in heterotrophic, mixotrophic or autotrophic cultures. The election of the best strategy will be based on different factors as species, number of transplantes required, or quality-price relationship.
Authors and Affiliations
Maria Jesús Cañal, Roberto Rodríguez, Belén Fernández, Ricardo Sánchez-Tames y Juan Pedro Majada
Isolation of a fragment of the ACC Oxidase cDNA expressed during the ripening of the Guava Enana fruit
Isolation and cloning of a cDNA fragment encoding ACC oxidase from ripe guava fruits by RT-PCR is shown in this report. It is the early step to apply RNA antisense technology for gene silence in order to control guava fr...
Minimal lethal concentration of hyrgromycin B in calli induction and shoot multiplication process of Digitalis purpurea L.
The plants of the genus Digitalis are characterized by the production of cardenolides, drugs widely used worldwide in the treatment of heart failure. In previous research a transformation protocol was developed from le...
Effect of applying an electromagnetic field on the soluble protein and carbohydrate content of embryos Coffea arabica L. zygotics grown in vitro
Electromagnetic fields have been applied to increase plant growth and in vitro germination of coffee. Even though it is not clear yet the mechanisms developed during the interaction of electromagnetic fields and plant...
Oxidative burst in plant-pathogen interaction
The production of reactive oxygen species (ROS) via consumption of oxygen in a so-called oxidative burst is a hallmark of successful recognition of infection and activation of plant defenses. ROS are not only toxic by-...
Use of Temporary Immersion Systems (RITA) in the propagation of plants via organogenesis in sugar cane and bananas
The present work had as objective to study the possibilities to use the RITA, in the micropropagation plant via axillary buds of sugarcane and of the banana hybrid (Musa sp. AAAA cv. FHIA-01 and AAAB cv. FHIA-18). Two d...