IN VITRO CULTURE OF MOSS BRYUM CORONATUM SCHWAEGR.(BRYACEAE) AND IT’S PHYTOCHEMICAL ANALYSIS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 9
Abstract
Objective: The purpose of the present investigation was to establish in vitro conditions for moss Bryum coronatum Schwaegr. And to carry out preliminary phytochemical screening of B. coronatum leaf extracts in different solvents.Methods: Fresh unopened, mature capsules were used as explant and surface sterilization of spores bearing capsule with different concentration of sodium hypochlorite (0.5, 1, 2, 4 and 8%) with different time duration. The MuÂrashige and Skoog (MS) medium that contains different concentration(full,1/2, 1/4th, 1/8th strength) with different concentration of sucrose were used for culture this moss. Phytochemical screening were carried out using ethanol, methanol and ethyl acetate leaf extract of B. coronatum to identify various constitutes using the standard procedures.Results: Surface sterilization of spores of this moss was most effective in 4% commercial bleach for 1 min sterilization. The optimum condition for germination of spores and for proper growth of gametophytes B. coronatum on MS/4 medium strength with sucrose (1.5%), at pH 5.8 and temperature 22±2ºC with 16/8h: light/dark condition. Phytochemical analyses revealed the presence of alkaloids, terpenoids and saponins in all extracts.Conclusion: Four percentage NaOCl aqueous solutions are better for surface sterilization of moss sporophytes. MS/4 medium with 1.5% sucrose found the best medium for spore germination. Solvents extracts showed presence of alkaloids, terpenoids and saponins in all extracts.Â
Authors and Affiliations
Vijay Kant Pandey, Rashmi Mishra, Ramesh Chandra
BIO-POTENTIATION AND IN VIVO PHARMACOKINETICS STUDIES OF POLYPHENOLS FROM PUNICA GRANATUM BY FORMING PHOSPHOLIPID CONJUGATES FOR BETTER ANTIOXIDANT & ANTI-INFLAMMATORY ACTIVITY
Objective: Polyphenols belong to the chemical class of flavonoids and are widely distributed in vegetables and plants. In this study, we aimed to potentiate the antioxidant and anti-inflammatory effects of polyphenols by...
NEW VALIDATED METHOD DEVELOPMENT FOR THE ESTIMATION OF SULFAMETHOXAZOLE AND TRIMETHOPRIM IN BULK FORM BY VISIBLE SPECTROSCOPY
Objective: To develop three novel, sensitive, simple validated visible spectrophotometric methods for the quantitative estimation of sulfamethoxazole (SMZ) and trimethoprim (TMP) in bulk form. Methods: Methods were base...
QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP ANALYSIS OF NOVEL PYRAZOLINE DERIVATIVES USING K NEAREST NEIGHBOUR MOLECULAR FIELD ANALYSIS METHOD
Objective: Malonyl CoA decarboxylase (MCD) enzyme plays important role in fatty acid and glucose oxidation. Inhibition of MCD might turn to a novel approach to treat ischemia. The main objective of this research article...
FLAVONOIDS FROM SUGAR BEET LEAVES AS HEPATOPROTECTIVE AGENT
Objective: This work was designed to investigate the activity of Beta vulgaris (B. vulgaris) extract against hepatotoxicity induced by (carbon tetrachloride) CC14 in male rats.Methods: Hepatoprotective study was performe...
A CONCEPT ON THE DEVELOPMENT OF BUPRENORPHINE AUTOINJECTOR FOR SELF AND EMERGENCY ADMINISTRATION
Objective: In natural and man-made disasters, pain and infection would increase morbidity and mortality. In these situations immediate treatment is mandatory and the drug has to be absorbed faster. An auto injector would...