In vitro Doses and Incubations Dependent Thrombolytic Potential Study of Edible Mushrooms Pleurotus ostreatus, Ganoderma lucidum and Lentinula edodes Available in Bangladesh
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 7, Issue 1
Abstract
Aims: The study was carried out to investigate the thrombolytic potential of methanolic extracts of edible mushrooms. Study Design: Experimental study. Place and Duration of Study: Department of Biochemistry, Primeasia University, August 2014 to December 2014 Methodology: An in vitro thrombolytic model was used to check the thrombolytic effect of Pleurotus ostreatus, Ganoderma lucidum and Lentinula edodes along with Streptokinase as a positive control and water as a negative control. In this study two concentrations of the extracts i.e. 400µg/ml and 800 µg/ml were tested at three time intervals, 24 hrs, 48 hrs and 72 hrs duration of incubation at 37ºC for observing maximum clot lysis. Results: At 400 µg/ml dose, Pleurotus ostreatus showed highest clot lysis activity in 72 hrs of incubation, 18.62% while Ganoderma lucidum, and Lentinula edodes showed 17.01% and 9.02% in the same incubation. Similarly, at 800 µg/ml dose, the thrombolytic activities were 63.35%, 34.16%, and 29.00% respectively for Pleurotus ostreatus, Ganoderma lucidum, and Lentinula edodes. Our study clearly demonstrated that Pleurotus ostreatus exhibits maximum 63.37% clot lysis at 800 µg/ml concentration in 72 hrs of incubation with reference to Streptokinase 86.43%. The thrombolytic nature of the mushroom extracts was found to be significant (P<0.0001), when compared with the negative control (water) at different doses. The relative thrombolytic activity of Pleurotus ostreatus, Ganoderma lucidum and Lentinula edodes was found to significant at different doses (P<0.0001) and at various incubation periods (P<0.0001). Conclusion: The result of our findings indicated that percentage of clot lysis is directly proportional to the concentration and incubation time of the extracts.
Authors and Affiliations
Maafi Rizwana Islam, M. Moyen Uddin Pk
Keywords
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- EP ID EP344268
- DOI 10.9734/BJPR/2015/18227
- Views 71
- Downloads 0
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