IN VITRO ESTABLISHING OF POPLAR AND WILLOW CLONES PERSPECTIVE FOR RENEWABLE ENERGETICS
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2017, Vol 9, Issue 1
Abstract
In vitro microclonal propagation of woody plants is an important biotechnological approach for their propagation. It allows to produce the clonal material of high quality and consequently to increase the productivity of plantations. For successful in vitro culture initiation of fast growing poplars and willow hybrids, the effectiveness of plants sterilization and survival were evaluated with the using various sterilization treatments and nutrient media. The buds from fast growing hybrids of willow clones: “Zhytomyrska-1”, “Lukash”, “Olimpiysky vohon”, “Pechalna” and poplars clones: “Lubenska” and “Kelibirdynska” were washed with different solutions – concentrated soap solution, sodium hypochlorite and 70% ethanol during different exposure time and then planted on nutrient MS and WPM media supplemented with different combinations of phytohormones. Highly efficient sterilization (78%) was achieved with the using three-step treatment of soap solution, sodium hypochlorite and 70% ethanol with exposure time of 2, 10 and 1 min respectively. When plants were cultivated on WPM media supplemented with 0.3 mg/l BAP and 0.1 mg/l IBA, high rate of rhizogenesis and induction of meristems were determined. Average rooting rate obtained in the research was 93%. All studied clones have been successfully established into in vitro culture, except poplar clone “Kelibrdynska”, what probably needs another protocol for cultivation. In vitro established plants will be later used for genetic engineering studies.
Authors and Affiliations
Л. В. ХУДОЛЄЄВА, Н. К. КУЦОКОНЬ, О. Г. НЕСТЕРЕНКО, Н. М. РАШИДОВ, О. М. ДУГАН
Keywords
Related Articles
- EP ID EP247004
- DOI -
- Views 91
- Downloads 0
Algal indication research in Ukraine
The development and establishing of the algal indicator focus of investigations in Ukraine are analyzed. The main achievements and results of work by algologists, hydrobiologists and ecologists in terms of the use of alg...
SEASONAL DYNAMICS OF HEAVY METALS CONTENT IN THE WELL WATER OF PRECARPATHIAN REGION
The purpose of our study was to analyze the annual dynamics of heavy metals content in drinking water of Precarpathian region and to compare it with the standards of heavy metals content in water. The object of our study...
BIOCHEMICAL FEATURES OF HEPATIC STEATOSIS UNDER THE ABSENCE OF VITAMIN A STORES
The work is devoted to the research of the characteristics of nonalcoholic hepatic steatosis and insulin resistance under the absence of vitamin A s.tores. To develop a vitamin A-deficient state transgenic mice were empl...
Peucedanum latifolium DC. (Аpiaceae Lindl.) у Чернівецькій області: хорологічні та екологічні особливості
Повідомляється про хорологічні особливості Peucedanum latifolium DC. – виду, що у Чернівецькій області потребує охорони на регіональному рівні. Відповідно до літературних джерел та гербарних зборів відомо вісім місцезнах...
ВПЛИВ СУБСТРАТІВ ВІДВАЛУ ВУГІЛЬНИХ ШАХТ НА ВМІСТ ПЕРОКСИДУ ВОДНЮ ТА АКТИВНІСТЬ ПЕРОКСИДАЗИ У SORGHUM HALEPENSE (L.) PERS
У роботі досліджено зміни вмісту пероксиду водню і активності пероксидази в 30- та 120-добових рослин Sorghum halepense (L.) Pers (довгокореневищного злаку родини тонконогових (Poaceae), який розмножується як насінням, т...