In vitro establishment of Bambusa vulgaris var. vulgaris Schrad. ex Wendl. at different times of the year
Journal Title: Biotecnología Vegetal - Year 2010, Vol 10, Issue 3
Abstract
Traditional propagation of Bambusa vulgaris var. vulgaris Schrad. ex Wendl is affected by low percentages of rooting and limited availability of propagules. These elements are handicaps for mass propagation of bamboos. Tissue culture offers a fast and reliable way to develop propagation protocols via organogenesis directly from axillary buds. The objective of this work was to determine the influence of the season to select the initial explant for the in vitro establishment. Two establishments were carried out each month during a whole year (24 at all). The number of buds sprouted and the number of explants free of visible microbial contaminants were quantified after 20 days of culture. Results showed that the season influenced on the in vitro establishment of B. vulgaris var. vulgaris. The highest numbers of buds sprouted (99%) and explants free visible of microbial contaminants (98%) was achieved between January to April and November-December.
Authors and Affiliations
Y. García-Ramírez , M. Freire-Seijo, Blanca Rosa Pérez, Ortelio Hurtado
Keywords
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