In vitro Evaluation of Antidiabetic and Anti-Inflammatory Activity of Ethanolic and Methanolic Extracts of Ceropegia juncea
Journal Title: Journal of Pharmaceutical Research International - Year 2017, Vol 15, Issue 2
Abstract
Antidiabetic and anti-inflammatory activity of ethanol and methanol extracts of in vivo and in vitro plants of Ceropegia juncea were evaluated by in vitro methods. Antidiabetic activity was studied using α-amylase inhibitory assay and α-Glycosidase inhibitory assay. In vitro anti-inflammatory activity was evaluated using albumin denaturation assay and membrane stabilization assay. Results showed that the in vivo and in vitro plant ethanol and methanol extracts exhibited significant activity with α-amylase inhibitory assay and α-Glycosidase inhibitory activity with IC50 values of 434.75±1.40, 578.16±0.09, 555.9±0.28, 748.35±0.58 µg/ml and 752.94±0.82, 797.17±1.02, 877.09±0.74 and 959.86±0.26 µg/ml respectively. The extracts also showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation and human red blood cell membrane (HRBC) stabilization with the IC50 values of 376±1.21, 372±0.92, 396±0.84, 344±01.08 µg/ml and 376±0.89, 910±1.06, 760±0.56, 810±0.12 µg/ml respectively. Thus, the both in vivo and in vitro plants of C. juncea could potentially be rich sources of natural antidiabetic and anti-inflammatory medicine. From the results, it is concluded that the metabolites present in vitro plant is almost similar to that of in vivo plant. So, in vitro plants can be used for medicinal purpose instead of in vivo plants.
Authors and Affiliations
M. Saraswathy, K. Kalimuthu, V. Chinnadurai, Y. Sharmila Juliet
Keywords
Related Articles
- EP ID EP312967
- DOI 10.9734/BJPR/2017/32020
- Views 99
- Downloads 0
Simultaneous Spectrophotometric Determination of Paracetamol and Dantrolene Sodium by Chemometric Methods
Aim: Simultaneous determination of Paracetamol (PAR) and Dantrolene Sodium (DAN) in pharmaceutical formulations was carried spectrophotometrically. Study Design: Two different chemometric techniques namely Partial Least...
In vivo Antioxidant Activity and Anti Hyperglycemic Relevant Enzyme Inhibition Properties of Petroleum Ether Extract of Traditionaly Processed Ocimum canum Leaves
Objective: To investigate the antihyperglycemic and antioxidant properties of the petroleum ether extract of Ocimum canum leaves in streptozotocin-induced diabetic rats. Place and Duration of Study: Pinacle Biomedical Re...
Patients’ Knowledge, Attitude and Compliance with Lifestyle Activities that can Potentially Interfere with the Outcome of Anti-hypertensive Medications
Non-drug therapy as component of management often compliments drug therapy in order to achieve positive clinical outcomes. This patient-focussed research studied 225 hypertensive patients comprising 95 males and 130 fema...
Prevention of Pioglitazone Induced Weight Gain by Co Administration of Piperine
Aim: Diabetes is the most common and one of the leading causes of mortality among non communicable diseases. Pilogitazone is the potent PPARr agonist used for the treatment of diabetes. The drug is associated with a subs...
Anti-inflammatory and Antinociceptive Effects of the Aqueous Extract of the Bark of Chrysobalanus icaco Linnaeus
Aims: This paper describes the anti-inflammatory and antinociceptive effects of the aqueous extract of Chrysobalanus icaco. Study Design: Study the anti-inflammatory and antinociceptive activity of the aqueous extract...