In vitro germination of Dendrocalamus strictus (Rosb.) Nees seeds
Journal Title: Biotecnología Vegetal - Year 2007, Vol 7, Issue 1
Abstract
The availability of D. strictus seeds is affected by the length and erratic flowering cycle of the species. These seeds also retain their germination only for short periods of time. The tissue culture offers a fast and reliable way for the propagation using in vitro seed germinated as initial plant material. This research was conducted with the objective of to germinate in vitro D. strictus seeds. The effect of different concentrations of sodium hypochlorite (NaClO) (3.0, 5.0 and 7.0%) and time for seed disinfection (20 and 30 min) were determined. It was evaluated the number of germinated seeds and free of microbial contaminants after 10 days of culture. The results showed that the method of disinfection which sodium hypochlorite 3.0% for 30 minutes was the best for in vitro germination of Dendrocalamus strictus (Rosb.) Nees seeds.
Authors and Affiliations
Yudith García-Ramírez , Marisol Freire-Seijo, Marisol Tejeda, Maritza Reyes
Keywords
Related Articles
- EP ID EP684083
- DOI -
- Views 126
- Downloads 0
Use of biological agents for phytosanitary control in the production of potato minitubers under acclimatization conditions
The employment of biological controls of pests and diseases is a technology with trends to increase at present for being economic, lasting and of beneficial results, related to the stability that they offer the ecosyste...
Protocol for Anthurium cubense Engler micropropagation from botanic seed
The popularity of Anthurium cubense, like pot plant, every day it is increased. Likewise, it has begun to extend the line of cultivated products for their employment as foliage. However, the traditional propagation of...
Effect of selenite and inulin on the Capsicum annuum L. - Phytophthora capsici interaction in greenhouse
Capsicum annuum L. is a crop of great economic importance in Mexico and is severely affected by the wilt caused by Phytophthora capsici. The objective of this work was to determine the effect of selenite and inulin on th...
Obtaining somatic embryos and establishing embryogenic cell suspensions in the banana cultivar 'Navolean' (AAB)
Cells suspension of plantains and bananas with promising results have been reported internationally, however, in Cuba it is not at so for AAB group. So, the following working objectives have to be considered: in vitro mu...
Effect of heat treatment at high temperature on in vitro seeds germination of Phaseolus vulgaris cv. ‘ICA Pijao’
The common bean (Phaseolus vulgaris L.) production is limited by biotic and abiotic factors. Among them, water stress, saline or high temperatures. To solve this problem, genetic improvement programs are aimed at the s...