In vitro Interaction between Fumonisin B1 and the Intestinal Microflora of Pigs
Journal Title: Polish Journal of Microbiology - Year 2017, Vol 66, Issue 2
Abstract
The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 µg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for the enumerating amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the amount of bacteria groups between the experimental (buffer, chyme, and fumonisin B1) and control 1 groups (buffer + chyme) were observed in both methods. Fumonisin B1 and hydrolysed fumonisin B1 concentration were analysed by liquid chromatograghy – mass spectrometry. There was no significant difference in FB1 concentration between the experimental and the control 2 group (buffer and fumonisin B1) at 0 h incubation, 5.185 ±0.174 µg/ml compared with 6.433 ±0.076 µg/ml. Fumonisin B1 concentration in the experimental group was reduced to 4.080 ±0.065 µg/ml at 24 h and to 2.747 ±0.548 µg/ml at 48 h incubation and was significantly less than that of in the control group. Hydrolysed fumonisin B1 was detected after 24 h incubation (0.012 ±0 µg/ml). At 48 h incubation time, hydrolysed fumonisin B1 concentration was doubled to 0.024 ±0.004 µg/ml. These results indicate that fumonisin B1 can be metabolised by caecal microbiota in pigs though the number of studied bacteria did not change.
Authors and Affiliations
Huu Anh Dang, Attila Zsolnai, Melinda Kovacs, István Bors, András Bónai, Brigitta Bóta, Judit Szabó-Fodor
Keywords
Related Articles
- EP ID EP251328
- DOI 10.5604/01.3001.0010.7858
- Views 93
- Downloads 0
Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion
Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the pla...
Evaluation of the Levels and Quality of Microbial Contamination in Medical Emergency Departments in Comparison to Other Workplaces
Work in Hospital Emergency Departments (HEDs) exposes both the emergency ward staff and patients to infectious and in other way harmful biological agents. The results of this study shows the presence of pathogenic bacter...
Study of Acid Phosphatase in Solubilization of Inorganic Phosphates by Piriformospora indica
Phosphorus is an essential plant macronutrient present in the soil. Only a small portion of phosphorus in soil is taken up by plants and the rest of it becomes unavailable to plants as it is immobilized. Phosphate solub...
Significance of meningococcal hyperinvasive clonal complexes and their influence on vaccines development
[i]Neisseria meningitidis[/i] is a commensal of human nosopharynx and humans are the only reservoir and host of this bacterium. It is also known as dangerous and devastating pathogen, which may lead to rapidly progr...
Isolation and Antimicrobial Testing of Aeromonas spp., Citrobacter spp., Cronobacter spp., Enterobacter spp., Escherichia spp., Klebsiella spp., and Trabulsiella spp. from the Gallbladder of Pigs
The presence of Gram-negative bacteria species, other than [i]Salmonella[/i] spp., in the gallbladder of pigs was examined. Isolated Gram-negative bacteria were assigned to species using the MicrogenTM GnA+B-ID Syst...