In Vitro Lethal Effect of Ellagitannins of the Fruit Rind of Punica Granatum and Stem and Root Alkaloids on The Miracidia and Cercariae of Schistosoma Mansoni
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
Ellagitannins of fruit rind and alkaloids of the stem and root of Punica granatum (P. granatum) were investigated for their in vitro lethal effect on miracidia, and cercariae of Schistosoma mansoni (S. mansoni). All the three investigated constituents were lethal to both Schistosome larvae at concentrations ranging between 0.39-50 ppm. At 0.39 ppm of the tested active constituents, the killing time of 50% of Schistosoma larvae ranged between 30.0 to 135 min, and 43 to 380 min, for miracidia and cercariae, respectively. While stem alkaloids were generally more active against cercariae, root alkaloids were more effective against miracidia. At 3.125 ppm, the LT100 for cercariae and miracidia were respectively, 50 and 120 min for the stem alkaloids, and 300 and 30 min for root alkaloids. Ellagitannins were also more effective against miracidia than cercariae. At a concentration of 6.25 ppm, ellagitannins killed 50% of the cercariae and miracidia after 42 and 3 min, respectively. Ellagitannins could be prepared cheaply from pomegranate fruit rind which is produced in large amounts during the commercial production of pomegranate juice. Therefore, ellagitannins could be used as an affordable method for the control schistosomiasis.
Authors and Affiliations
Khalid H. Abu Zeid, Mohamed F. El-Badawy, Saad A. Gumaa, 4, Ahmed Ismael, Mohamed M. Shohayeb
Keywords
Related Articles
- EP ID EP387016
- DOI 10.9790/3008-1106048088
- Views 90
- Downloads 0
Continuous Flow Injection Analysis- Precipitation Reaction Of Ibuprofen With Sodium Nitro Pros Side Using Low Pressure Mercury Lamp Tube (UV-Light) And Detection Of Diverged Scattered Lights (Visible Light) At 2 X 90o Using Multi Solar Cells That Covers 2 X 100mm Distance Of 2mm Path Length.
A new, simple, fast and sensitive method has been developed for the determination of Ibuprofenin pure form and drugs (tablets)by continuous flow injection scatter light. The method was based on the reaction of the Ibupro...
Validated Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometric Method for Quantification of Milnacipranin Human Plasma for Pharmacokinetic Study: ARandomized, Open-Label, Two-Period, Comparative Crossover Study in Healthy Korean Male Volunteers
A sensitive and specific liquid chromatography combined with electrospray ionization (ESI) tandem mass spectrometry (LC-MS/MS) method, operating in the positiveionization mode, for quantifying of milnacipran in human pla...
Electrophoretic Profile of Protein and Isozymes in Leaf Gall Of Pongamia Pinnata (L.)
In the present investigation the electrophoretic studies of protein and isozymes (peroxidase and polyphenol oxidase) in leaf galls of Pongamia pinnata induced by Aceria pongamiae were carried out. The results showed some...
Factors Affecting Biodegradation of the Organophosphorus Insecticide Diazinon by Bacterial Mono-Culture of Bacillus Sefensis 7, Isolated From the Rhizosphere of Date Palm Tree
Due to widespread use pesticides for crop protection, they are considered as contaminants in the, environmental matrices such soil which are exposed to large quantities. Three isolates from soil samples using enrichment...
Investigation of Some Coagulation Parameters in Pregnant Womens with Preeclampsia
Background/Aim: Pre-eclampsia (PE) is a major obstetric health problem in developing countries and considers as one of the main causes of maternal mortality. The study aimed to investigate changes in some coagulation par...