In Vitro Maturation of Goat Oocyte with Deferent Hormonal Additives and in Vitro Fertilization by Buck and Ram Sperm
Journal Title: PARIPEX-Indian Journal of Research - Year 2016, Vol 5, Issue 8
Abstract
This study included the evaluation hormonal additive for tissue culture media in vitro maturation (IVM)and fertilization (IVF) of goat. With the trail hybrid fertilization of doe oocytes by ram semen. Therefore 910 oocytes collected by slicing and aspiration from goat ovary included oocytes complete surround with oocytes partial surrounded by cumulus cells , and 25 oocytes recovered for hybrid fertilization . Hormonal additive ( LH ,FSH ,estrogen ,progesterone ,and total mixed) in the tissue culture media .The best result when used the mixed of total hormones additive in the TCM199 through in vitro maturation and fertilization, when the oocytes recovered by slicing or aspiration . While the progesterone used in the TCM199 gives low IVM ,and IVF in both slicing or aspiration of oocytes source .The hybrid between doe oocytes with the ram semen ,only three oocytes reached the embryonic development
Authors and Affiliations
Saad Akram Hatif, Omar Mezher abdulla
Keywords
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