In vitro multiplication of Morinda royoc L. in Temporary Immersion Systems
Journal Title: Biotecnología Vegetal - Year 2011, Vol 11, Issue 2
Abstract
Morinda royoc L. is a medicinal plant which has identified numerous secondary metabolites important for medical and pharmaceutical industry. The use of in vitro culture techniques could contribute to the production of these. The aim of this work was to multiply M. royoc using Temporary Immersion System (TIS). It was used TIS of 1000 ml capacity. Each contained 250 ml MS medium with 4.4 μM benzyladenine (BA) and 2.9 μM indole acetic acid (IAA). The SIT were inoculated with 30 individual explants (shoot tips and nodal segments). It was determined the effect of the immersion frequency (two, four and six immersions of two minutes per day) and the type of explants (shoot tips, nodal segments) on the multiplication of shoots and biomass production. It was found that with four and six immersions per day the highest values of multiplication coefficient and shoot length were obtained. The maximum biomass production was achieved with six immersions per day. No hyperhidricity symptoms were observed in shoots. It was noted that the nodal segments produced more shoots per explant, increased multiplication coefficient and biomass than the apex, while the latter resulted in longer shoots.
Authors and Affiliations
Elio Jiménez , Carlos Reyes , Pablo Machado , Naivy Pérez-Alonso , Alina Capote , Anabel Pérez , Bettina Eichler-Loebermann
Keywords
Related Articles
- EP ID EP683760
- DOI -
- Views 135
- Downloads 0
Scale up Pseudomonas fluorescens-based biofertilizers production
In previous studies, a semi-synthetic culture medium was optimized for the industrial production of a plant growth-promoting strain of Pseudomonas fluorescens. This work was proposed to develop the scale up for producing...
In vitro organogenesis in Digitalis genus
The genus Digitalis have special interest due to the therapeutic properties of its secondary metabolites, known generically as cardenolides. These compounds are commonly used in the treatment of cardiovascular disease....
Somatic embryos formation in five Theobroma cacao L. cultivars grown in Venezuela
One of the main problems of cocoa (Theobroma cacao L.) is the observed variability in plantation from seeds that affecting the yield and quality of grain. This has led to considered asexual propagation (cuttings and gr...
In vitro multiplication of Morinda royoc L. in Temporary Immersion Systems
Morinda royoc L. is a medicinal plant which has identified numerous secondary metabolites important for medical and pharmaceutical industry. The use of in vitro culture techniques could contribute to the production of...
Effect of the application of AIA and sucrose in the in vitro rooting of the varieties Sonate and Lambada of Anthurium andraeanum Lind.
The effect of different concentrations of indole-3-acetic acid (AIA) and sucrose Anthurium andraeanum in the varieties ‘Lambada’ and ‘Sonate’ in the enraizamiento phase during the micropropagation of this specieswas de...