In vitro Pharmacological Properties and Phenolic Contents of Stem Barks Extracts of Piliostigma reticulatum (DC) HOCHST and Piliostigma thonningii (SCHUM) MILNE-REDH. (Caesalpiniaceae)
Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 17, Issue 2
Abstract
Aims: This study aimed to investigate the cytotoxic, antioxidant and lipoxygenase inhibition activities of extracts from stem barks of Piliostigma reticulatum and Piliostigma thonningii. Methodology: Methanol extract and decoction from stem barks of P. reticulatum and P. thonningii were evaluated for their lipoxygenase inhibitor effect and antioxidant properties using 2, 2’-azino-bis (acid 3-ethylbenzothiazoline-6-sulfonique (ABTS) and lipid peroxidation. Cytotoxic effect on leukemia cell lines (K562) was evaluated by trypan blue assay. Total phenolic, tannins and flavonoids contents in the extracts were determined with standard methods. Results: P. thonningii and P. reticulatum methanol extracts inhibited lipoxygenase activity with IC50 values of 7.80 ± 0.57 and 12.86 ± 1.47 µg/mL respectively. With ABTS scavenging method, P. thonningii methanol extract showed the strongest activity with IC50 value of 3.96 ± 0.03 µg/mL, followed by P. reticulatum methanol extract (IC50 = 4.90 ± 0.07 µg/mL). Both decoctions from these plants had scavenged ABTS+ radical. Methanol extracts from P. thonningii and P. reticulatum exhibited significant activity against lipid peroxidation and K562 cells viability. Phytochemical analysis showed the presence of phenolic compounds including tannins and flavonoids in methanolic extracts and decoctions from P. reticulatum and P. thonningii. Conclusion: The extracts from Piliostigma reticulatum and Piliostigma thonningii stem barks exhibited lipoxygenase inhibitor, antioxidant and cytotoxic effects.
Authors and Affiliations
Ouédraogo Noufou, A. M. Emmanuel Thiombiano, Sawadogo W. Richard, Tibiri André, Martin Kiendrebeogo, Lompo Marius, Diederich Marc, Guissou I. Pierre
Keywords
Related Articles
- EP ID EP349662
- DOI 10.9734/EJMP/2016/30417
- Views 100
- Downloads 0
Hepatoprotective Activity of Rubia tinctorum’s Extract against CCl4 Induced Hepatic Injury in Rats
This study investigated the hepatoprotective activity of Rubia tinctorum’s extract against CCl4 induced liver damage in rats. Twenty four rats were divided into four groups. Group I received normal saline (10 ml/kg) and...
Antioxidant Capacity of Phenolic from Seed Extracts of Lagenaria siceraria (Short-Hybrid Bottle Gourd)
Aims: To evaluate the antioxidant components and antioxidant activities of seed extracts of Lagenaria siceraria (Short-Hybrid Bottle Gourd). Study Design: In vitro assessment of antioxidant assays; quantitative determina...
Modulation of Cytochrome C Release and Opening of Mitochondrial Permeability Transition Pore by Calliandra portoricensis (Benth) Root Bark Methanol Extract
Aim: Evasion of apoptosis, a form of programmed cell death, is a common feature of most cancer cells. This study evaluated the modulatory effects of different fractions of crude methanol extract of the root bark of Calli...
Cytotoxic, Antioxidant and Apoptotic Effects of Twenty Sri Lankan Endemic Plants in Breast Cancer Cells
Aims: Main aim of this study was to evaluate cytotoxic, antioxidant and apoptotic properties of Sri Lankan endemic plants in estrogen receptor positive (MCF-7) breast cancer and triple negative breast cancer (MDA-MB-231)...
Phytochemical and Anti-diarrhoeal Properties of Methanolic Leaf Extract of Maerua crassifolia Forssk
Maerua crassifolia Forssk. Leaf is used in African traditional medicine for management of gastrointestinal disorders. The anti-diarrhoeal activity of the methanol extract of Maerua crassifolia leaf was investigated in ra...