Cytotoxic, Antioxidant and Apoptotic Effects of Twenty Sri Lankan Endemic Plants in Breast Cancer Cells
Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 15, Issue 1
Abstract
Aims: Main aim of this study was to evaluate cytotoxic, antioxidant and apoptotic properties of Sri Lankan endemic plants in estrogen receptor positive (MCF-7) breast cancer and triple negative breast cancer (MDA-MB-231) cells. Place and Duration of Study: At the Institute of Biochemistry, Molecular Biology and Biotechnology, between June 2013 and May 2015. Methodology: We investigated leaf and bark extracts (hexane, chloroform, ethyl acetate, methanol) of twenty endemic plants for possible cytotoxicity against breast cancer (on estrogen receptor positive MCF-7 and triple negative MDA-MB-231 breast cancer) cell lines and normal mammary epithelial cells (MCF-10A). Cytotoxic extracts were also investigated for possible apoptotic effects on MDA-MB-231 cells by caspase 3/7 estimation. Total polyphenols, flavonoids and the free radical scavenging activity of the extracts were also measured. Results: Of the 156 solvent extracts from 20 plants studied only 35 extracts from 14 plants were significantly cytotoxic [18 extracts to MCF-7 cells (IC50 31.43 to 97.50 µg/mL), 7 extracts to MDA-MB-231 cells (IC50 16.11 to 95.83 µg/mL) and 10 extracts (IC50 0.51 to 99.35 µg/mL) to both cell lines] with only some of these being less cytotoxic to normal breast epithelial cells. Caspase 3/7 was activated by some of the cytotoxic extracts. Polyphenol and free radical scavenging activity were high in most of the methanol and ethyl acetate extracts. Flavonoids were present in all the extracts in varying quantities. Conclusion: The cytotoxic and apoptotic potential demonstrated by some plants endemic to Sri Lanka, indicates strong leads for future drug discovery for combating breast cancer.
Authors and Affiliations
Panchima P. Jayarathna, Kamani H. Tennekoon, Sameera R. Samarakoon, Ira Thabrew, Eric H. Karunanayake, E. Dilip de Silva, Meran K. Ediriweera
Keywords
Related Articles
- EP ID EP349671
- DOI 10.9734/EJMP/2016/26591
- Views 90
- Downloads 0
Biological Activities of the Hydro-alcoholic and Aqueous Extracts of Achillea falcata L. (Asteraceae) Grown in Jordan
Aim: This study aimed to screen the aqueous and hydro-alcoholic extracts of Achillea falcata L. (Asteraceae) grown in Jordan for their antioxidant, antibacterial, antiplatelet and anti-proliferative efficacy. Study Desig...
The Antimicrobial and Phytochemical Analysis of the Leaves of Aspilia africana on Clinical Isolates
The uses of medicinal plants for treatment of various infections in traditional communities have been an age-long practice. This provides the rationale to study medicinal plant extracts as a possible source of alternativ...
Phytochemical Analysis of Plectranthus sp. Extracts and Application in Inhibition of Dental Bacteria, Streptococcus sobrinus and Streptococcus mutans
Aims: Evaluation of leaves methanol extracts from Plectranthus barbatus and Plectranthus ecklonii (Lamiaceae) against oral pathogens. Place and Duration of the Study: P. barbatus and P. ecklonii, cultivated in Botanic Ga...
Analgesic, Anti-inflammatory and Other Pharmacological Activities of Methanol Extract of Rhododendron campanulatum from Nepal
Aims: Rhododendron campanulatum D. Don is a plant of Ericaceae family that is found at an altitude of 3000-4200 meters. Traditionally, it has been used to cure rheumatism, hemicranias, sciatica and colds. This study aims...
Elemental and Fatty Acid Content of Four Medicinal Plants: Kaiempferia rotunda, Cuscuta reflexa, Centella asiatica and Asparagus racemosus
Aims: The present study investigates the elemental and fatty acid content of four different medicinal plants, namely Kaiempferia rotunda, Cuscuta reflexa, Centella asiatica and Asparagus racemosus, grown in Bangladesh. M...