In vitro Propagation of Oreocallis grandiflora (Lam.) R. Br., A Medicinal Threatened Plant
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 14, Issue 3
Abstract
Aims: To promote the conservation and biotechnology approach of Oreocallis grandiflora, a promising wild, medicinal and agroforestry Peruvian plant, a methodology for its in vitro propagation was performed. Study Design: Disinfection and germination of seed, followed by shoot multiplication and rooting using in vitro techniques. Place and Duration of Study: Follicles were collected in Yupa - Huaraz (9°30'26.76"S, 77°28'21.33"W) and the experimental procedure was done between January 2013 and December 2016. Methodology: Follicles with different colors were disinfected with ethanol 70% at different times, and seeds were place on basic culture medium composed by ½ Murashige and Skoog, sucrose (30 g•L-1) and phytagel (3 g•L-1), at pH 5.7. Shoot induction was performed using N6-benzylaminopurine (BAP) at 0.5, 1, 2, 3 and 4 mg•L-1. For root induction, α-naphthalenacetic acid (NAA) or gibberellic acid (GA3) at 0.25, 0.50, 0.75 and 1.0 mg•L-1 were used. Results: Disinfection of follicles at 15 and 20 min. of 70% ethanol were successful at 100%, and all seed germinated without addition of any growth regulator. The best shoot induction treatments were 3.0 and 4.0 mg•L-1 of BAP at 60 days of PGR exposition. For the root development PGR supplement was not necessary, but GA3 improved rooting percentage and explants growth while NAA induced callusing. Conclusion: This is the first report of in vitro propagation of O. grandiflora, and provides important results to promote its domestication, ex situ conservation, and biotechnological applications.
Authors and Affiliations
Percy Olivera-Gonzales, Edson M. Yldefonzo, Elder M. Mestanza, Carmen Tamariz-Angeles
Keywords
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- EP ID EP313444
- DOI 10.9734/ARRB/2017/34724
- Views 98
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