IN VITRO PROTECTIVE EFFECT OF EXTRACTS OBTAINED FROM VARIOUS SANSEVIERIA SPECIES AGAINST OXIDATIVE DAMAGE OF PROTEINS IN EQUINE ERYTHROCYTES
Journal Title: Słupskie Prace Biologiczne - Year 2017, Vol 1, Issue 14
Abstract
The aim of this study was to evaluate in vitro the effect of buffer extracts obtained from leaves of various species from Sansevieria genus against protein damage in equine erythrocytes. The leaves of Sansevieria francisii Chahin, S. caulescens N.E.Br., S. suffruticosa N.E.Br., S. roxburghiana Schult. & Schult.f., S. metallica Gérôme & Labroy, S. gracilis N.E.Br., S. hyacinthoides (L.) Druce, S. cylindrica Bojer ex Hook., S. canaliculata Carrière, S. aethiopica Thunb., S. kirkii Baker, S. trifasciata Prain, S. forskaliana (Schult. & Schult.f.) Hepper & J.R.I.Wood, S. fischeri (Baker) Marais, S. dooneri N.E.Br., S. intermedia N.E.Br., S. parva N.E.Br. were sampled for study. Freshly collected leaves were washed, weighted, crushed, and homogenized in 0.1M sterile phosphate buffer saline solution (pH 7.4) (in proportion 1:19, w/w) at room temperature. The extracts were then filtered and investigated for their antioxidant activity. Blood was drawn from jugular vein of the horses involved in horseback riding, in the morning, 90 minutes after feeding. Blood was stored into tubes with sodium citrate and held on the ice until centrifugation at 3000 rpm for 5 min. The pellet of blood was re-suspended in sterile 4 mM phosphate buffer (pH 7.4). A volume of 0.1 ml of the various extracts was added to 1.9 ml of clean equine erythrocytes. As positive control phosphate buffer was used. After incubation the mixture at 37°C for 60 min with continuous stirring, samples were used for biochemical assay. To evaluate the protective effects of extracts obtained from leaves of various species of Sansevieria genus against free radical-induced protein damage in equine erythrocytes, a carbonyl derivatives content of protein oxidative modification (OMP) assay based on the spectrophotometric measurement of aldehydic and ketonic derivatives in the erythrocytes’ suspension was performed. When erythrocytes were incubated with leaf extracts of various species from Sansevieria genus, the aldehydic derivatives level was significantly reduced by 13.6% (p<0.05) for S. forskaliana extract. Moreover, all extracts (except S. francisii extract) reduced the formation of intracellular aldehydic derivatives of OMP in the extracts-treated erythrocytes, but these results were non-significant. Treatment by extracts of various Sansevieria species reduced the concentration of ketonic derivatives of OMP, when compared to untreated erythrocytes. The most potent effect was demonstrated by the S. canaliculata, S. forskaliana, S. aethiopica, S. cylindrica, S. metallica, S. hyacinthoides, and S. kirkii compared to phosphate buffer saline control (16.1%, 14.7%, 13.4%, 12.9%, 12.9%, 12.7%, 12.1%, respectively). However, there were no significant changes for other extracts. The experimental evidence obtained in the present study indicates that various species from Sansevieria genus are a rich source of compounds that manifest antioxidant activity. Moreover, various species from Sansevieria genus can effectively protect erythrocytes against oxidative damage. Thus, S. canaliculata, S. forskaliana, S. aethiopica, S. cylindrica, S. metallica, S. hyacinthoides, and S. kirkii may be a valuable source of natural antioxidants that may potentially be recommended for applications in medicine and veterinary practice. The present findings suggest that the extracts of various species from Sansevieria genus have shown remarkable potential in protecting the protein groups and reducing the protein carbonyl content. According to the abovementioned antioxidant mechanisms, extracts of various species from Sansevieria genus may inhibit formation of protein carbonyl by scavenging free radicals formed in vitro. According to many supporting documents, it can be assumed that secondary plant metabolites, i.e. polyphenolic compounds in extracts of various species from Sansevieria genus extract may contribute to the antioxidant activity.
Authors and Affiliations
Halyna Tkachenko, Lyudmyla Buyun, Paweł Pażontka-Lipiński, Marlena Witaszek, Zbigniew Osadowski
Keywords
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