In vitro rapid propagation of Kaempferia elegans
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 7
Abstract
The shoot bases of Kaempferia elegans sterile seedlings were used as explants which were subcultured for propagation, induced for multiple shoots, and rooted to produce rooted plants, and their medium for tissue culture were screened to establish a rapid propagation system for K. elegans. The results showed that the appropriate medium for propagation of K. elegans were MS+6-BA 8～12 mg/L+NAA 0～0.1 mg/L+coconut milk 10%+sucrose 30 g/L+cara rubber powder 10 g/L, and the best propagation medium was MS+6-BA 8 mg/L+NAA 0.1 mg/L+coconut milk 10%+sucrose 30 g/L+cara rubber powder 10 g/L. The suitable medium for inducing multiple shoots were MS+6-BA 3～5 mg/L+NAA 0.05~0.1 mg/L+ coconut milk 10%+ sucrose 30 g/L+ cara rubber powder 10 g/L, and the most suitable medium for inducing multiple shoots was MS+6-BA 3 mg/L+NAA 0.05 mg/L+ coconut milk 10%+ sucrose 30 g/L+ cara rubber powder 10 g/L. The optimum rooting medium was MS+ NAA 0.1 mg/L+ mashed banana 10～15%+activated carbon 0.5～1.0 g/L+ sucrose 30 g/L+ cara rubber powder 9.5 g/L.
Authors and Affiliations
Man-liang WU, Dong-mei LI
Keywords
Related Articles
- EP ID EP600988
- DOI 10.16768/j.issn.1004-874X.2018.07.005
- Views 48
- Downloads 0
Response of starch RVA spectrum of different varieties of rice to exogenous selenium fertilizer
The purpose of this paper is to study the RVA spectrum of starch of different rice varieties treated with exogenous selenium. The completely random design was used, at high altitude mountain laboratories（800 m）above, d...
Histocytological observation and chlorophyll syntheses characterization of chimera variety of Ananas bracteatus
The histocytological features and chlorophyll biosynthesis process were studied to illustrate the structural and physiological basis of the chimera variety formation mechanism in Ananas bracteatus. The histocytological o...
Influence of Bacillus subtilis strain TR21 wettable powder on the banana rhizosphere microorganisms
In order to study effects of TR21 wettable powder on soil microorganism in banana rhizosphere, ten rhizosphere soil samples from different planting stage treated by TR21 wettable powder and water control were collected,...
Process and quality of low temperature pressing hemp seed oil
This paper studied the extraction technology of hemp seed oil using low temperature hydraulic pressing method. Firstly, to screen the raw materials of hemp seed, and then the effect of time, temperature, pressure and mat...
Study on the pathogenicity diversity of Xanthomonas oryzae pv. oryzicola from Guangdong province
Rice disease resistance is the result of interaction between host and pathogen. The knowledge of differentiation of pathogenic strains is the prerequisite for disease resistance evaluation and its application. In this st...