In Vitro Regeneration of Banana Cv. Nanjanagud Rasabale (AAB) by Shoot Tip Culture
Journal Title: Biotechnology Journal International - Year 2017, Vol 20, Issue 4
Abstract
The cultivar Nanjanagud Rasabale (Musa spp. AAB, Silk subgroup) geographical indication tagged and leading cultivar of Mysore district. It is known for unique taste, has a huge demand across the country. In vitro regeneration in banana is preferred for faster multiplication rate compared to sucker propagation. The present study was carried out with the objective to investigate the effect of different antioxidants, BAP and auxins on in vitro regeneration of banana cv. Nanjanagud Rasabale. Shoot tip explants cultured on MS liquid medium supplemented with ascorbic acid 50 mg/l was found effective in controlling browning. Enhanced shoot proliferation and shoot growth were observed with MS medium containing BAP 4.0 mg/l + NAA0.50 mg/l. In vitro rooting of microshoots was effective with half strength MS medium supplied with NAA 0.5 mg/l.
Authors and Affiliations
Prabhuling Guranna, Rashmi Hegde, Babu Ammanaghatta Govindaswamy
Keywords
Related Articles
- EP ID EP315067
- DOI 10.9734/BJI/2017/39892
- Views 106
- Downloads 0
Influence of Type and Age of Primary Somatic Embryo on Secondary and Cyclic Somatic Embryogenesis of Cassava (Manihot esculenta Crantz)
This study investigated the influence of age of the cotyledons, cut from primary somatic embryos (PSE) developed from shoot meristems (SM) or immature leaf lobes (LL), on secondary somatic (SSE) and cyclic (CSE) embryoge...
Apoptotic Gene Expression in Sheep Hepatocytes during Fasciola hepatica Infection (Fascioliasis)
Aims: The main objective of this work was to investigate the apoptotic genes of sheep liver hepatocytes to elucidate the apoptosis pathway mechanisms during Fasciola hepatica infection using molecular and serological tec...
Biovalorization of Olive Mill Waste Water for the Production of Single Cell Protein from Saccharomyces cerevisiae, Candida utilis and Pleurotus ostreatus
Aims: The aim of this work was to investigate and optimize the potential of olive mill waste water (OMWW) to be utilized as a substrate for the production of single cell protein (SCP). Study Design: The study was divided...
Screening of Aromatic Rice Cultivars for Bacterial Leaf Blight (BLB) Disease Resistance Gene Xa5 through Molecular Marker
Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most common devastating diseases of rice (Oryza sativa) all around the world. Thus, present study has been conducted to screen 12 ar...
Characterization of New Quality Protein Maize (QPM) Varieties from a Breeding Program: Analysis of Amino Acid Profiles and Development of a Variety-Diagnostic Molecular Marker
Aims of the Study: This study was to determine the amino acid profile of two newly developed quality protein maize varieties and to develop variety-diagnostic molecular markers. Methodology: Two new maize varieties, name...