In vitro Shoot Tip Response to Encapsulation-Dehydration Procedure for Cryopreservation of Philippine Taro [Colocasia esculenta (L.) Schott]
Journal Title: International Journal of Research in Agricultural Sciences - Year 2018, Vol 5, Issue 2
Abstract
This study was conducted to explore the feasibility of the encapsulation-dehydration cryopreservation procedure and provide the groundwork for establishing a cryopreservation protocol for routine application in germplasm conservation of Philippine taro. Shoot tips (2-3mm) from 2-3 week old in vitroplantlets of VG-2taro variety cultured on modified Murashige and Skoog (MS) medium were used. Initial experiments determined the effects of pretreatment (liquid MS with 0.15M or 0.3M sucrose), encapsulation (3% sodium alginate in MS solution and 0.1M calcium chloride in MS or distilled water), preculture (24h on MS with 0-1.2M sucrose) and dehydration with silica gel (3h or 4h) on tissue viability to ensure viable tissues before liquid nitrogen (LN) immersion. Results showedthat only the high-sucrose preculture affected tissue viability in terms of reduced number of shoot-forming explants and delay in shoot initiation. In the encapsulation-dehydration experiments, the same pretreatment and encapsulation treatments as above, 12-24h preculture in MS with 0-1.0M sucrose, dehydration in silica gel for 3.5h (about 20% moisture content), 1-2d LN immersion, thawing in 40oC water bath for 3 min, and regrowth in MS+5% sucrose or MS+3% sucrose+0.2 mg/L kinetin+0.2 mg/L benzyladenine, were tested. These treatments had no remarkable effect on post-thaw tissue survival. More than 80% of the shoot tips remained green after thawing. After 3d in the regrowth medium, 28-33% of the shoot tips remained green but there was no sign of shoot growth. Lack of regrowth indicates cryopreservation failure and this defines future research toward developing a successful protocol.
Authors and Affiliations
V. Z. Acedo, et al.
Keywords
Related Articles
- EP ID EP501661
- DOI -
- Views 76
- Downloads 0
Nest Architecture and Colony Characteristics of Meliponula bocandei(Hymenoptera, Apidae, Meliponini) in Cameroon
There are several hundred species of stingless bees existing worldwide, which differ significantly in colour, body and colony size. In Africa stingless are distributed throughout the tropical and subtropical parts where...
Assessment of Faba Bean Gall Disease Intensity in North Shoa Zone of Central, Ethiopia
Faba bean (Vicia faba L.) is one of the earliest domesticated food legumes in Ethiopia and is now cultivated on large areas in many countries. It serves as an important role in the socio-economic life of the farming comm...
Studies on Sensors for Measuring Soil Compaction for Effective Crop Production-A Review and Analysis
The compaction of soil due to repeated field operation for many years makes the soils an impermeable layer called soil hardpan. The introduction of heavy machinery is also responsible for creating soil hard pan. A great...
Udder Morphology and Machine Milking Ability in Dromedary Camels
Udder and teatmorphology is an important factor in the machine milkability of Dromedary camels.However,the morphological characteristics of the udder and their relation to machine milkability in D...
Cassava Production and Options of Sales Outlets in Oyo State
To ensure continuity in cassava production, the farm household has an option of selling to the general market or to the cassava processing industries about which little research have been done. The study therefore focuse...