IN VITRO STUDIES ON CALLUS INDUCTION OF ECLIPTA ALBA (L.) HASSK: AN IMPORTANT PLANT OF TRADITIONAL MEDICINE
Journal Title: Journal of Biological and Scientific Opinion - Year 2018, Vol 6, Issue 3
Abstract
An efficient method was developed for rapid and large-scale in vitro clonal propagation of the valuable medicinal herb Eclipta alba (Asteraceae). Different explants were tried, and best results were obtained by axillary shoot proliferation in cotyledonary nodal segments. The explant type, medium composition, various carbon sources, plant growth regulators markedly influenced in vitro propagation of Eclipta alba. An in vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium. Callus profuse growth was obtained on MS medium augmented with 2,4-D (1.0 mg/l) and BAP (0.5 mg/l). This combination was selected to maintain the callus. BAP (1.0 mg/l) and NAA (0.1 mg/l) showed maximum shooting (16-20). Rooting was highest on full strength. Micropropagated plants were transferred to a mixture of garden soil, farmyard soil, and sand (2∶1∶1). These plants grew normally without showing any morphological variation.
Authors and Affiliations
Shikha Bhansali, Ashwani Kumar
Keywords
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- EP ID EP651118
- DOI 10.7897/2321-6328.06384
- Views 80
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