In vitro Study of the Survival, Reproduction and Morphology of Daphnia pulicaria irradiated with a Low Energy Laser
Journal Title: Journal of Nuclear Physics, Material Sciences, Radiation and Applications - Year 2018, Vol 6, Issue 1
Abstract
Daphnia is a genus of crustaceans that is representative of freshwater communities. The species exhibit a high sensitivity to a wide range of toxic compounds so that they have been used internationally as biomonitors in toxicity tests to evaluate ecosystem conditions such as water quality. It is also a model genus in genetics, epigenetics and reproductive ecology. In this work, we used Daphnia pulicaria as a model to measure the effects of low-energy laser irradiation on survival, reproduction, and morphology variables of parental organisms and their offspring. We used (1) a single clone line of organisms to eliminate interindividual genetic variability; (2) individuals from more than 50 generations after the clone line was established, and offspring from the third brood onwards to dissipate maternal and epigenetic effects, and (3) neonates, those individuals of the species that have less than 48 hours of life, because they are the most sensitive stage to optical stimuli. We analyzed number of deaths, longevity, age at first reproduction, number of offspring per week, number of total offspring during all their life cycle, body size, size of the antennules, and length of the apical spine of the 4th and 5th brood of the irradiated individuals, who were exposed to a blue laser stimulus of 405 nm for 25 minutes with a power of 40 mW at a distance of 50 cm, compared to those of the control (non-irradiated) group.
Authors and Affiliations
F. Zaldivar , J. Montoya , S. Gonzalez , L. A. Mandujano , J. F. Mendez-Sanchez , L. Romero , J. Mulia , M. Paulin , D. Osorio-Gonzalez
Keywords
Related Articles
- EP ID EP596767
- DOI 10.15415/jnp.2018.61019
- Views 222
- Downloads 0
Electrochemical Characterization of Cathodized Copper Oxide Thin Films
Cathodic electrodeposited copper oxide thin films were prepared using 0.1 M aqueous (distilled water) copper sulphate solution onto the surface of stainless steel substrates. Deposited samples were characterized for stru...
Temperature Effects in the Composition of Metal Halide Perovskite thin Films
Metal halide perovskites have shown to be a structure with great promise as an efficient photovoltaic, but at the same time it is affected by instability problems that degrade their performance. Degradation mechanisms va...
Studies on Neutron, Photon (Bremsstrahlung) and Proton Induced Fission of Actinides and Pre-Actinides
We present the yields of various fission products determined in the reactor neutron, 3.7-18.1 MeV quasi-mono energetic neutron, 8-80 MeV bremsstrahlung and 20-45 MeV proton induced fission of 232Th and 238u using radioch...
Partial as Well as Total Photon Interaction Effective Atomic Numbers for Some Concretes
Photon interaction effective atomic number (Zeff) for partial as well as total photon interaction processes has been computed using logarithmic interpolation method for seven different concretes viz. (i) Ordinary, (ii) H...
Potential of Some Fungal and Bacterial Species in Bioremediation of Heavy Metals
Microorganisms including fungi and bacteria have been reported to extract heavy metals from wastewater through bioaccumulation and biosorption. An attempt was, therefore, made to isolate bacteria and fungi from sites co...