In Vivo and In Vitro Anti-trypansomal Evaluation of Crude Methanolic Extracts of Crotalaria albicaulis and Cistanche phelypaea against Trypanosoma evansi
Journal Title: UNKNOWN - Year 2019, Vol 9, Issue 4
Abstract
Camel trypanosomiasis is a major disease of economic importance in Ethiopian Somali Regional State, Ethiopia. Local people use the leaves of Crotalaria albicaulis and Cistanche phelypaea to treat this disease and complement the absence or the limitations of existing anti-trypanosomal chemotherapeutic agents. Thus, the aim of this study was to evaluate the in vivo and in vitro anti-trypanosomal activity of the methanolic crude extracts of leaves of Crotalaria albicaulis and Cistanche phelypaea against Trypanosoma evansi isolate. The fresh leaves of both plants were extracted by percolation technique using methanol. For the in vivo assay, mice infected with T. evansi were administered intraperitoneally daily for 7 days with crude extracts at doses of 100, 200 and 400 mg/ kg body weight. In vitro trypanocidal activity of these extracts was tested in triplicate in 96 well micro titer plates. Diminazene aceturate and dimethyl sulfoxide were used as positive and negative controls, respectively. At 4.0 mg/ ml concentration the methanolic crude extract of C. albicaulis completely ceased motility of the parasite after 40 minutes. The plant also significantly (p<0.05) prolonged infection period in vitro infectivity test. Moreover, at 400 mg/kg dose, the crude extracts of both plants exhibited a mild in vivo anti-trypanosomial activity against T. evansi. This study established that leaves of C. albicaulis has a mild in vivo and in vitro anti-trypanosomal activity and can be considered as a potential source of new drugs for the treatment of Camel trypanosomiasis.
Authors and Affiliations
Markos Tadele, Getachew Alebie, Yitagesu Tewabe
Keywords
Related Articles
- EP ID EP670215
- DOI -
- Views 45
- Downloads 0
EGFR Tyrosine Kinase Targeted In Silico Design and Synthesis of Novel Quinazoline Derivatives
Quinazolines are medicinally important as anti-convulsant, anti-cancer, anti-microbial, and anti-tubercular properties etc. They target epidermal growth factor receptors on tumor cells. This work is aimed to synthesize a...
Novel Chiral Chalcone Analogs that Induce M Phase Arrest and Apoptosis in HeLa Cells
We identified two chiral chalcone analogs CCL360 and CCL361 from the Chiba Chemical Library, which exhibit antiproliferative effects on human cancer cells. Flow cytometry analyses revealed that HeLa and Vero cells treate...
Synthesis and Biological Evaluation of Novel 1,4-Disubstituted 1,2,3-Triazoles and Bis 1,2,3-Triazoles as Anti-Bacterial Agents
A library of novel 1,4-disbustituted 1,2,3-triazoles 3a-3k was prepared by using Click-chemistry concept. In this 1,3-dipolar cycloaddition, the 3-methoxy-4-(prop-2-yn-1-yloxy)benzaldehyde (1) was used as alkyne partner...
A Short Review on Biological Potential Thiopyridazine Analogues
Pyridazinone/thiopyridazinone derivatives represent one of the most active classes of compounds possessing a wide spectrum of biological activities such as cardiovascular properties, anti-inflammatory, analgesic, antidia...
Taste Receptor Tas2r5 and Tas1r3 is Expressed in Sublingual Gland
Type 2 taste receptors (TAS2Rs), a large family of GPCRs, were first discovered in the gustatory system, and are co-expressed in a subset of taste receptor cells and detect bitter-tasting compounds. Type 1 taste receptor...