In Vivo and In Vitro Anti-trypansomal Evaluation of Crude Methanolic Extracts of Crotalaria albicaulis and Cistanche phelypaea against Trypanosoma evansi
Journal Title: UNKNOWN - Year 2019, Vol 9, Issue 4
Abstract
Camel trypanosomiasis is a major disease of economic importance in Ethiopian Somali Regional State, Ethiopia. Local people use the leaves of Crotalaria albicaulis and Cistanche phelypaea to treat this disease and complement the absence or the limitations of existing anti-trypanosomal chemotherapeutic agents. Thus, the aim of this study was to evaluate the in vivo and in vitro anti-trypanosomal activity of the methanolic crude extracts of leaves of Crotalaria albicaulis and Cistanche phelypaea against Trypanosoma evansi isolate. The fresh leaves of both plants were extracted by percolation technique using methanol. For the in vivo assay, mice infected with T. evansi were administered intraperitoneally daily for 7 days with crude extracts at doses of 100, 200 and 400 mg/ kg body weight. In vitro trypanocidal activity of these extracts was tested in triplicate in 96 well micro titer plates. Diminazene aceturate and dimethyl sulfoxide were used as positive and negative controls, respectively. At 4.0 mg/ ml concentration the methanolic crude extract of C. albicaulis completely ceased motility of the parasite after 40 minutes. The plant also significantly (p<0.05) prolonged infection period in vitro infectivity test. Moreover, at 400 mg/kg dose, the crude extracts of both plants exhibited a mild in vivo anti-trypanosomial activity against T. evansi. This study established that leaves of C. albicaulis has a mild in vivo and in vitro anti-trypanosomal activity and can be considered as a potential source of new drugs for the treatment of Camel trypanosomiasis.
Authors and Affiliations
Markos Tadele, Getachew Alebie, Yitagesu Tewabe
Keywords
Related Articles
- EP ID EP670215
- DOI -
- Views 59
- Downloads 0
Convenient Synthesis and Structural Characterization of a Series of Methyl 4-[2-(cyclized amino)ethoxy]benzoate Esters as Key Intermediates in Synthesis of Selective Estrogen Receptor Modulators.
Estrogen receptors, the important selective targets in management of serious aspects in women health challenging issues, guided the scope of drug discovery towards developing new entities treati...
Polydopamine for Biomedical Application and Drug Delivery System
The aim of this paper is to evaluate the properties of polydopamine and its application in medicine and pharmacy. A dark brown-black insoluble biopolymer produced by the autoxidation of dopamine is poly...
Newcastle Disease Virus: Structural and Molecular Basis of Pathogenicity
Newcastle disease is highly contagious infection caused by Newcastle Disease Virus also designated as Avian Paramyxovirus serotype-1. The pathogenicity differs according to the strain however; the molecular b...
The Potential Inhibitors in Traditional Chinese Medicine for BCR-ABL T315I Mutation of Chronic Myelogenous Leukemia
Chronic Myelogenous Leukemia (CML) is a myeloproliferative disorder characterized by the appearance of abnormal proliferation of white blood cells in the Philadelphia chromosome. Current drugs target ABL kinase may have...
Antimicrobial, α-Glucosidase and Alkaline Phosphatase Inhibitory Activities of Bergenin, The Major Constituent of Cissus populnea Roots
Bergenin (1) was isolated as the major compound of the Roots of Cissus populnea. Its structure was elucidated on the basis of spectral analysis, including 1D and 2D NMR experiments and Mass Spectrometry (MS) as well as b...