In vivo Anti-trypanosomal Evaluation and Phytochemical Analysis of Methanol Extract of Nauclea latifolia
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 13, Issue 2
Abstract
Aim: Trypanosomiasis is a pathological condition that requires serious attention. The dried root of Nauclea latifolia was investigated to determine the anti-trypanosomal activity on groups of Wistar rats. Methods: A 1000 mg/kg body weight of methanol extract was administered for 7 consecutive days by intraperitoneal route (ip) to Group II while Group I received a 3.5 mg/kg of Samorinil® through the same route. The phytochemical constituents were also determined. Parasitaemia level, body weight, temperature and packed cell volume (PCV) were monitored and determined before and after the commencement of the experiment. Results: The packed cell volume (PCV) increased significantly (P = .05) in Group I and II post infection from an average of 35.0 ± 0.68 to 38.05 ± 0.32% when compared to the infected - untreated group (Group III) with a PCV value of 30.0 ± 1.11%. The methanol extract at a concentration of 1000 mg/kg of the plant reduced the parasitaemia level from 7.8 to 2.53 trypanosomes per ml at the end of the experiment. In Group I, the positive group, that received commercial drug (diminazene diaceturate (Samorinil®) was aparasitic before the end of the experiment. There was significant weight improvement (p =.05) in the entire Group (I and II) from ̶ 22 ± 0.01 to 4.4 ± 0.30 g and ̶ 2.4 ± 0.20 to 4.1 ± 0.04 g respectively except the Group III that was untreated with ̶ 2.4 ± 0.34 to ̶ 6.6 ± 0.18 g that kept depreciating postinfection. First death was recorded in Group III on 12-day post-infection. Temperature of G III continued to increase slowly as the parasitaemia level increased from 35.22 to 42 .36°C which significantly varied (p =.05) from the rectal temperature of GI and II from 35.85°C and 35.17 to 35.53°C and 34.40°C. Conclusion: The experiment showed that a 1000 mg/kg methanol extract of Nauclea latifolia exhibited promising trypanocidal activity against T. brucei.
Authors and Affiliations
C. E. Ugwu, I. O. Ezeh, N. C. Obitte, S. A. Chime, C. O. Agubata
Keywords
Related Articles
- EP ID EP342187
- DOI 10.9734/BJPR/2016/27249
- Views 108
- Downloads 0
Public Knowledge and Awareness about Gout: A Cross-sectional Study in Qatar
Background: The prevalence of hyperuricemia and gout had shown an increasing trend all over the world including in developing countries. The patients may receive very little education on the dietary and lifestyle factors...
A Prospective Study for “Developing School Based Model for Mainstreaming Rational Use of Medicine”
Background and Objectives: For mainstreaming rational use of medicines (RUM), consumer education is a must. It was felt that a school based model, developed to empower teachers regarding RUM using a modular programme, ca...
Synergistic Activity of the Antibiotic Meropenem in Combination with Edible Mushroom Extracts against Multidrug Resistant Bacteria
Aims: The objective of this study was to evaluate the antibacterial potency of meropenem in synergism with five edible mushroom extracts against multidrug resistant clinical isolates. Methodology: Minimum Inhibitory Con...
Free Radical Scavenging Activity of the Nigerian Leech (Aliolimnatis michaelseni) Saliva Extract
Introduction: Antioxidant is a substance that is capable of restricting or frustrating the oxidation of other oxidizable molecules by suppressing the free radical- caused oxidation chain reaction. They occur naturally in...
Design, Formulation and Tableting Properties of Aqueous Leaf Extract of Moringa oleifera
Aim: The aim of this study is to formulate a standard dose of aqueous extract of Moringa oleifera leaves into tablets and to determine a suitable binder for the formulation. Methodology: Aqueous extract...