In vivo Antiplasmodial Activity and GCMS Analysis of Vernonia colorata (Willd) Drake Leaf
Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 14, Issue 3
Abstract
Aim: To analyse the phytochemical components using GCMS technique and evaluate the antiplasmodial activity of crude extract and partitioned fractions of Vernonia colorata leaf. Study Design: Qualitative, gravimetric and Chromatographic methods of analysis were adopted for phytochemical analysis, while experimental design (In vivo) was used for antiplasmodial study. Place and Duration of Study: Department of chemistry and Biochemistry, Federal University of Technology Minna Niger State, Nigeria, between April 2014 to April 2015. Methodology: The crude extract and partitioned fractions were evaluated for their antiplasmodial activity in vivo against Plasmodium bergei at 200, 400 and 600 mg/kgbw using standard methods. The combined fractions obtained after column chromatography were analysed using GCMS analysis. The antiplasmodial activity of V. colorata in mice infected with P. berghei showed effects of graded doses of the extract with dose dependent inhibition of parasitaemia with maximum effect at 600 mg/kg. The oral median lethal dose (LD50) in mice was also evaluated and estimated to be greater than 5000 mg/kg. GCMS analysis was carried out on combined fractions (VC20) of V. colorata leaf. Results: The analysis showed major chemical constituents such as 9octadecenoic acid (12.86%), 3, 11tetradecadien1ol (19.90%), 13docosenoic acid (13.86%) and 4methyl1decene (24.07%). The antiplasmodial evaluation carried out in this research shows a considerable antiplasmodial activity of V. colorata.
Authors and Affiliations
Muhammad Hauwa Idris, Abdullahi Mann, Adamu Yusuf Kabiru, Musa Bola Busari
Keywords
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- EP ID EP349704
- DOI 10.9734/EJMP/2016/18826
- Views 104
- Downloads 0
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