In-Vivo Evaluation and Comparison of Ileal Digestibility of Indispensable Amino Acids (IAA) of Formulated High Protein Cookies in Normal and Uremic Rats
Journal Title: International Journal of Health Sciences and Research - Year 2017, Vol 7, Issue 8
Abstract
Objective: The aim of this study is to formulate the high protein cookies incorporated with egg white powder and to determine the ileal digestibility of Indispensible amino acid for the formulated high protein cookies in uremic female wistar rats. Methods: Female wistar rats of age 8-12 weeks were selected for the study. The rats were grouped as control and uremic group. Nephrotoxicity was induced in wistar rats by administration of gentamicin 80 mg/g/day intraperitoneally. The rats were fed with cookies for 14 day period. Each day each rat received its diet for 10 minutes period as nine meals given at hourly intervals (0800-1600 hour). On the 14th day, from 5-7 hours, the rats were asphyxiated with carbon dioxide and decapitated. The 20 cm of ileum immediately anterior to ileocaecal junction was dissected out and ileal contents were flushed using distilled water, freeze dried and subjected to chemical analysis. Results: The formulated cookie and ileal digesta were estimated for indispensible amino acid using high performance liquid chromatography. It was found that the rate of absorption of phenylalanine and methionine were found to be higher in uremic rats compared to the control group. The other indispensible amino acids were poorly absorbed at the terminal ileum in uremic rats showing significant decrease in absorption. Conclusion: From the study it is concluded that the protein absorption is better in uremic rats and hence it can be recommended for subject with kidney disease. Hence kidney disease subjects should consume adequate high biological value protein to compensate the protein loss.
Authors and Affiliations
Girija K
Keywords
Related Articles
- EP ID EP373942
- DOI -
- Views 47
- Downloads 0
Contributing Factors Associated with Multi-Drugs Resistance Tuberculosis among MDR-TB Clients of Kathmandu, Nepal
ntroduction: The emergence of Multi-Drug Resistant Tuberculosis (MDR-TB) has become a significant public health problem and an obstacle to effective TB control measure. Objective: The objective of this study was to asses...
Non-Albicans Candida among Symptomatic Vulvovaginal Candidiasis Women in Onitsha, Nigeria
Background: Non-albicans Candida (NAC) species have emerged as important causes of Vulvovaginal candidiasis (VVC) among women of reproductive age. The aim of this study was to establish an association between NAC and sym...
Assessment of Life Satisfaction among Cerebrovascular Accident Patients
Life satisfaction is the way persons evaluate their lives and how they feel about their directions and options for thefuture. It is a measure of well-being and may be assessed in terms of mood, satisfaction with relation...
Keratoderma Hereditariamutilans: A Rare Disorder of Keratinization
Keratoderma hereditariamutilans is a rare, autosomal dominant disorder of keratinization characterized by palmoplantar keratoderma and ainhum-like constrictions. We report herein a 16-year-old deaf –mute girl, since birt...
Effect of Singing along with Pulmonary Rehabilitation on Quality of Life and Dyspnea in Patient with Chronic Obstructive Pulmonary Disease
Introduction: Chronic obstructive pulmonary disease (COPD) patients have many symptoms like dyspnea, cough, fatigue, etc. The Global Initiative for chronic obstructive Lung Disease has categorized these patients accordin...