In vivo Germination of Encapsulated Orchid Seeds of- Epidendrum radicans
Journal Title: Asian Journal of Biotechnology and Bioresource Technology - Year 2017, Vol 2, Issue 1
Abstract
The orchid seeds are naked embryos without any nutritive tissue to support them for germination in vivo. In nature, they germinate symbiotically when they come in contact with mycorrhiza found in the mother root tufts. Orchid capsule consists of hundreds or thousands of seeds which go waste without germination. The role of mycorrhiza is to convert starch into simple glucose which provides nutrition for the germinating seeds. Orchid breeders use in vitro technique to culture the orchid seeds with various growth hormones. An attempt was made to grow orchid seeds in vivo by encapsulating them with calcium alginate similar to the production of synthetic seeds. Epidendrum radicans seeds were encapsulated with calcium alginate. The sodium alginate gel was prepared using Vacin and Went medium supplemented with 100 ml L-1 CW, 2 mg L-1 NAA, and 20 g L-1 sucrose. The encapsulation was carried out aseptically and the seeds were successfully germinated in the sterilized soilrite medium.
Authors and Affiliations
Grace Leena Crasta, A. K. Bopaiah
Keywords
Related Articles
- EP ID EP337922
- DOI 10.9734/AJB2T/2017/35852
- Views 66
- Downloads 0
Assessments of Medicinal Plants Used in Treatment of Various Ailments in Kano City, Nigeria
Aim: The aim of this research is to conduct an Ethno botanical assessments of medicinal plants used in the treatment of various ailments in Kano metropolis, Nigeria Methodology: A systematic survey of medicinal plants, u...
Dynamics of Micro/Macro Elements and Heavy Metals during Anaerobic Treatment of Organic Fraction of Municipal Solid Waste (OFMSW)
In this study, the dynamics of macro and micro elements (including heavy metals) during anaerobic treatment of organic fraction of municipal solid waste (OFMSW) was investigated to determine the effect of seasonal variat...
Anaerobic Digestion of Abattoir Waste: A Combined Strategy for Biogas and Biofertilizer Production, and Waste Management
Aims: The study was carried out to estimate the biogas and biofertilizer potential of cattle Paunch and assess the waste treatment efficiency of the Anaerobic Digestion process. Place and Duration of Study: The study was...
Microbial Load, Prevalence and Antibiotics Susceptibility of Bacteria Isolated From Naira Notes
Money is a means of exchange for goods and services. It develops from sales, salaries, ceremonies, compensations, etc. The microbial load, prevalence and antibiotics resistance of bacterial population isolated from the e...
Studies on Physicochemical Analysis and Biodecolorization Potential of Some Bacteria Isolated from Textile Effluent
Aim: This research work is aimed at studies on physicochemical analysis and biodecolorization potential of some bacteria isolated from textile effluent. Study Design: This study is designed to isolate and identify specie...