In vivo investigations of neurotensin receptors in adipocytes, hepatocytes and enterocytes of rat
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2011, Vol 18, Issue 2
Abstract
Introduction: Atherosclerotic vascular disease is currently the biggest threat and the highest cause of death worldwide, approaching almost 60%.The development of atherosclerosis is affected by ecological factors associated with industry and pollution of the environment. Neurotensin (NT) is a peptide acting via 3 kinds of neurotensin receptors (NTR) localized in target tissues. In several studies, the presence of its receptors has been shown in chicken liver, and the influence of NT on the metabolism of this organ was confirmed (glycogenolysis stimulation through sympathetic nervous system, entero-hepatic circulation of bile acids, metabolism of lipoproteins).Materials and methods: Healthy male WISTAR rats weighing 300±30 grams, were used for the experiments. The animals were divided into 4 groups: 1) control group, to which 0.9% NaCl was administrated (i.p.); 2) the second group was given levocabastine 1mg/kg i.p.; 3) the third group received SR 48692 0.4mg/; 4) the fourth group was given NT analog [D-Trp 11]-neurotensin 15nM/kg. Plasmatic membranes of liver, small intestine and adipose tissue were prepared according to the method of Havrankova. Analysis of results obtained in the investigation of NT receptors was performed using the Scatchard method from LIGAND-Pc, v. 3.1 software.Results: The investigation of antigenity of I 125 NT showed proper antigen-antibody reaction. No binding of the I 125 NT with plasmatic membranes of adipocytes or enterocytes was observed. Unspecifi c binding of I 125 NT with 10 μmol/L of free NT was observed in the plasmatic membranes of hepatocytes.Conclusion: The presence of NT receptors only in the membranes of hepatocytes may suggest their role in the regulation of lipid metabolism via receptor – ligand way.
Authors and Affiliations
Paweł Maćkowiak, Jacek Piątek, Hanna Krauss, Dorota Nowak
Keywords
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