Incidence of infections with extended spectrum beta Lactamase (ESBL)-producing gram-negative bacteria among patients admitted in medical intensive care unit of tertiary care hospital
Journal Title: Pathology Update: Tropical Journal of Pathology and Microbiology - Year 2017, Vol 3, Issue 2
Abstract
Introduction: Extended spectrum beta-lactamases (ESBLs) are beta-lactamases capable of conferring bacterial resistance to the Penicillins, first, second and third generation cephalosporins, and Aztreonam (but not the Cephamycins or Carbapenems). Clinicians, Microbiologists and hospital epidemiologists are concerned about ESBL-producing Gram negative bacteria because of increased incidence of such infections, limitations of effective antimicrobial therapy and adverse patient outcomes. Aims and Objectives: To find the incidence of infections caused by ESBL producing Gram negative bacteria among patients admitted in Medical ICU of tertiary care hospital. To compare the antimicrobial susceptibility patterns of the ESBL producing Gram negative isolates with ESBL non-producing isolates. Materials and Methods: Clinical samples were collected from patients admitted in Medical ICU & processed as per standard protocol. Antibiotic susceptibility was performed by Kirby-Bauer disc diffusion method. Screening for ESBL production was done as per CLSI guidelines. Presence of ESBL was detected by Phenotypic confirmatory disc diffusion method using discs containing 30µg of Ceftazidime with and without 10µg of Clavulanic acid on Mueller-Hinton Agar. Results: A total of 50 samples were collected. Of these, majority (38%) of samples were urine followed by sputum (36%). Of the 50 samples, majority of the isolates were Klebsiella spp and E.coli, 14(28%) each. Among 50 samples, 42(84%) isolates were ESBL producers. Of the 42 ESBL producing organisms, majority(13(30.9%)) of them were Klebsiella spp. Conclusion: In the present study, we found an alarming number(84%) of ESBL producing Gram negative isolates in Medical ICU patients. Strict antibiotic policies and measures to limit indiscriminate use of Cephalosporins should be undertaken to minimize the emergence of such resistance.
Authors and Affiliations
Ganesh Perumal P, Jnaneshwara KB, Asha B. Patil, Akshay R
Keywords
Related Articles
- EP ID EP263234
- DOI 10.17511/jopm.2017.i2.17
- Views 130
- Downloads 0
Study of fine needle aspiration cytology of lymphadenopathy in tertiary care centre of Ahmedabad, Gujarat
Introduction: Lymph nodes are a site for organized collections of lympho-reticular tissue and are pink gray bean shaped encapsulated organs. Lymphadenopathy is one of the most common clinical presentations of patients at...
Role of p16, Ki67 and CK17 in differentiating benign lesions, cervical intraepithelial neoplasia(CIN) and atypical immature squamous metaplasia(AIM) of uterine cervix
Cervical intraepithelial neoplasia (CIN) is a premalignant lesion capable of progressing to cervical cancer. Despite the existing well-defined criteria, the histopathological diagnosis is subject to high rates of discord...
Role of imprint cytology and frozen section in the diagnosis of tumours
Introduction: Imprint cytology is an economical, simple and quick method with excellent cytological clarity but cannot provide information on the depth of invasion. Frozen section is the standard method of intraoperative...
Pathological and Microbiological Analysis of Cerebrospinal Fluid in Bacterial Meningitis
Background: Meningitis is a life threatening infection with incidences increasing in adults and children. CSF analysis is a key tool to diagnose and differentiate between bacterial, viral, tubercular and fungal meningiti...
A clinicopathological study of salivary gland tumors
Background:To evaluate tumors involving major and minor salivary glands, histopathology is an essential diagnostic method and is the gold standard. This study was carried out to know the incidence of salivary gland tumor...