Incidence of multiple drug resistant Bacillus cereus in some popular snacks and sweets sold in Kolkata city, India
Journal Title: Indian Journal of Microbiology Research - Year 2017, Vol 4, Issue 1
Abstract
Background: Snacks and sweets from road side eateries are cheap sources of nutrition but occasionally cause numerous public health hazards most importantly gastroenteritis. Various factors including poor infrastructure to prepare foods, post-preparation contamination during storage and handling as well as unhygienic surroundings may contaminate these foods with pathogenic bacteria. Bacillus cereus is one of the common and important food borne pathogens causing spoilage to the foods and producing gastroenteritis to the consumers. This study was carried out to enumerate B. cereus and assess their spoilage potentiality and antibiotic resistance pattern. Methods: Standard plate count was performed using plate count agar while presumptive B. cereus was isolated in Bacillus cereus agar base supplemented with polymyxin B selective supplement and egg yolk emulsion. Confirmation as B. cereus was done by morphological, biochemical and physiological methods. Confirmed isolates were tested for extracellular enzyme production and antibiotic resistance pattern. Result: Standard plate count exceeded 1010 cfu g-1 and B. cereus count was positive in 57% of the samples. All the 43 B. cereus isolates produced amylase and caseinase and were multiple drug resistant. None produced lipase. Conclusion: Presence of B. cereus with spoilage potential and multiple drug resistance require good manufacturing practices (GMPs) and good hygienic practices (GHPs) during food production and storage.
Authors and Affiliations
Arindam Roy, Jyotismita Khasnabis, Paulomi Adhikari, Deblina Chowdhury, Chandan Rai
Keywords
Related Articles
- EP ID EP293564
- DOI 10.18231/2394-5478 .2017.0003
- Views 69
- Downloads 0
Extent of antimicrobial resistance in Acinetobacter species in a Tertiary Care Teaching Hospital
Acinetobacter, gram negative coccobacillus, has become a frequent pathogen in hospitals and other health care settings. Acinetobacter species cause a wide variety of illness in debilitated and hospitalized patients espec...
Incidence & Estimation of Beta – Lactamase Enzymes (ESBL Ampc, Carbapenemase Enzymes) singly and their coexistence in clinical isolates of Gram Negative bacteria by Vitek – 2 Compact System
Introduction: With increase in Beta – Lactamases producing microbial infections there is need for rapid method for identification of different types of Beta Lactamases enzyme production & their coexistence in clinical is...
Introduction of a modified interactive tutorial in Microbiology among II nd Phase MBBS students
Tutorials are very significant methodology and usually used as a method of reinforcement of teaching learning process. Tutorials have mostly become teacher-led and students usually remain passive. There is a need to modi...
An epidemiological study of Tinea capitis in patients attending a tertiary care hospital
Background: Tinea capitis (scalp ringworm) is the most common dermatophyte infection of the scalp affecting mainly children and rarely adults. The epidemiology of Tinea capitis varies within different geographical areas...
Prevalence and antibiotic susceptibility pattern of multidrug resistant gram negative bacilli in lower respiratory tract Infections in a tertiary care hospital of Tripura
Introduction: Lower respiratory tract infections with multidrug resistant gram negative bacteria are hard to treat and cause high morbidity and mortality. This study gives an account of isolation of multidrug resistant b...