INCREASED URINARY 8-OXO-7,8-DIHYDRO-2′-DEOXYGUANOSINE EXCRETION IN A SAMPLE OF EGYPTIAN CHILDREN WITH BETA THALASSEMIA MAJOR: MARKER FOR LIPID PEROXIDATION-INDUCED DNA DAMAGE
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2017, Vol 10, Issue 12
Abstract
Objective: The objective of this research was to evaluate oxidative stress status in children with β-thalassemia major.Methods: Our study was conducted in children with β-thalassemia aged from 5 to 15 years. Investigate the urinary excretion of human 8-oxo-7,8- dihydro-2′-deoxyguanosine, which will be analyzed by enzyme-linked immunosorbent assay. To investigate serum levels of antioxidant enzymes include glutathione s-transferase (GST) and catalase (CAT).Results: We found a significant elevation of the urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine level with p=0.001 compared to control group, a significant reduction of both GST and CAT p=0.05 and 0.03, respectively, compared to control group. There was a significant negative correlation between urinary 8-oxo-7,8-dihydro-2′-deoxyguanisine and CAT level, r=−0.378, p=0.016, hemoglobin - r=−0.610, p=0.001, hematocrit (%) - r=−0.478, p=0.002, while a significant positive correlation between urinary 8-oxo-7,8-dihydro-2′-deoxyguanisine and alanine aminotransferase - r=0.547, p=0.001, and serum ferritin - r=0.391, p=0.013. There was a significant negative correlation between CAT and serum ferritin - r=−0.320, p=0.44.Conclusion: We conclude that the strongly increased urinary excretion 8-oxo-7,8=dihydro-2′-deoxyguanisine indicates elevated lipid peroxidation induced DNA damage in internal organs such as the liver. These highly pro mutagenic lesions may contribute to the increased risk of thalassemia patients to develop hepatocellular carcinoma.
Authors and Affiliations
Mona A Elabd, Dina Abu Zeid, Marwa A Elhady, Maged A El Wakeel, Ghada M El-kassas, Rania N Sabry, Eman Awadallah
Keywords
Related Articles
- EP ID EP605521
- DOI 10.22159/ajpcr.2017.v10i12.21045
- Views 99
- Downloads 0
FORMULATION AND EVALUATION OF NOVEL ANTIBACTERIAL AND ANTI-INFLAMMATORY CREAM CONTAINING MUNTINGIA CALABURA LEAVES EXTRACT
  Objective: The main objective of the present study was to formulate antibacterial and anti-inflammatory cream using Muntingia calabura leaves extract.Methods: 500 g of matured leaves of M. calabura were extracted wit...
ESTIMATION OF ENTRANCE SURFACE RADIATION DOSE TO THYROID REGION IN COMPUTED TOMOGRAPHY BRAIN EXAMINATION
Objective: The objective of this study was to estimate the entrance surface radiation dose to the thyroid region in a computed tomography (CT) brain scan.Methods: Unfors Multi-O-Meter equipment was used to measure the en...
N-ACETYLCYSTEINE, A BOON FOR YELLOW PHOSPHORUS-INDUCED ACUTE LIVER FAILURE? A CASE REPORT
Rat killer, in the form of a paste, is a commonly used rodenticide in India. It contains 3% yellow phosphorus, which is a local and a systemic toxin that damages all tissues it contacts. The lethal dose of yellow phospho...
IN SILICO DOCKING ANALYSIS OF YOHIMBINE ALKALOIDS FROM CATHARANTHUS ROSEUS AGAINST DENGUE FEVER
Objective: In this docking study was performed by the active site of NS2B-NS3 protease with selected plant alkaloids derived from Catharanthus roseus. On the basis of docking results, out of the nine alkaloids yohimbine...
ACTIVITY OF INDIAN TOPICAL SKIN PHARMACEUTICALS AGAINST SOME FUNGAL PATHOGENS
 Objective: The aim was to assess the in vitro antifungal activity of some locally available pharmaceutical preparations used to cure skin diseases. Mostcommonly used preparations were evaluated for dermatophytic and no...