Indicators of cellular immunity in case of рassalurosis of rabbits
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2019, Vol 35, Issue
Abstract
Introduction. Researching the characteristics of immunity is the best way to effective prevent and treat the diseases. Helminthiasis immunity has a number of features that are caused by relationships in the host-parasite system. Passalurosis is quantitatively dominant among many types of rabbit helminths on the globe. The goal of the work was to determine the influence of Passalurus ambiguus on indicators of cellular immunity of rabbits. Materials and methods. Analog groups of male rabbits of 3-5 months old were selected for the experiments. Intensity of invasion was determined by the method of the Mac-Master. The count of T- and B-lymphocytes was determined by the method of spontaneous rosette-formation with sheep erythrocytes. Rabbits with pasalurosis had different levels of invasion intensity (II): low (II = 276.47±43.33 eggs in 1 g of feces), high (II = 2446.67±422.11 eggs in 1 g of feces) – II and medium (II =1293.75±275.80 eggs in 1 g of feces) – III research groups. We did not find helminth’s eggs in the control group. Results of research and discussion. It was found that in the blood of rabbits with pasalurosis in groups I, II, and III, compared with healthy ones, there was significantly high number of lymphocytes by 11.48% (p <0.05), 17.37% (p <0.01), 11.31% (p <0.05), due to both B-lymphocytes (1.74 times (p <0.001), 1.96 times (p <0.001), 2.20 times (p <0.001), T-lymphocytes (1.17 times (p <0.05), 1.22 times (p <0.001), 1.28 times (p <0.001)), against the background of a low percentage of O-lymphocytes by 13,81% (p <0.001), 15.22% (p <0.001), 16.83% (p <0.001). Probably the increased number of T-lymphocytes in infected animals was observed in rabbits with a high content of T-helpers in the blood, which were 1.25 times (p <0.01), 1.41 times (p <0.001) and 1.60 times (p <0.001) higher compared with the control group. In addition, the total number of T-active blood lymphocytes was 1.37 times (p <0.01), 1.54 times (p <0.001), and 1.74 times (p <0.001) higher, respectively, in animals of the І, II and III groups than in the control one. Such a change in the subpopulation composition of T-lymphocytes indicated the activation of the protective mechanisms of the rabbit organism in response to mechanical damage to the epithelium and metabolic products of the pathogen Passalurus ambiguus. Conclusions. In general, the results of studies showed that effect of the pathogen Passalurus ambiguus in the blood of rabbits leaded to an increase in T- and B-lymphocytes, T-helper cells and T-active ones, against the background of a decrease in О-lymphocytes. Such redistribution of lymphocyte populations is an immune response to parasitism of рassalurіs.
Authors and Affiliations
Y. V. Duda, M. P. Prus
Keywords
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- EP ID EP659987
- DOI 10.31073/vet_biotech35-05
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