Indirect Shoot Organogenesis in Jatropha Curcas (L) for in Vitro Propagation
Journal Title: PARIPEX-Indian Journal of Research - Year 2015, Vol 4, Issue 12
Abstract
Jatropha curcas a monoecious perennial shrub belongs to family euphorbiaceae. Mainly known as a biodiesel crop its commercialization is elusive due to variations in seed yield, oil content, and slow germination rates. Optimization of in vitro conditions for callusing and rapid regeneration is of paramount importance due to its slow regeneration. We used different concentrations of growth hormones followed by optimizing the conditions that gave best results on MS medium supplemented with IBA (3mg/l) + KN (1mg/l). Callus regeneration was tried on different concentrations and combinations of BAP + KN + GA3. Regeneration of shoot primordial was highest in the calli cultured on MS + BA (2 mg/l) + KN (3 mg/l). Shoots were grown after 4-5 weeks of incubation under optimized conditions. This developed protocol would be utilized for long scale production of elite genotypes and for genetic interventions of Jatropha curcas. This in vitro protocols provides the usage for rapidly establishing high oil yielding plantlets.
Authors and Affiliations
Manali Gangwar, Swati Sharma, Rajinder Singh Chauhan, Hemant Sood*
Keywords
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