Indirect somatic embryogenesis of Swietenia macrophylla King in semisolid culture medium
Journal Title: Biotecnología Vegetal - Year 2010, Vol 10, Issue 3
Abstract
Biotechnological techniques are an alternative to propagate Swietenia macrophylla King. However, micropropagation by organogenesis in this species has been little or not developed due to big microbial contamination and low indexes of in vitro plants regeneration. Therefore, somatic embryogenesis plays an important role in the propagation of this woody species. Immature cotyledons were used as initial plant material to establish a protocol of indirect somatic embryogenesis. Different growth regulators and their concentrations were used to develop the indirect somatic embryogenesis stages. The use of a semisolid culture medium composed of MS salts with 2,4-dichlorophenoxyacetic acid (4.0 mg l -1 ) favored the formation of callus in the explants. The highest percentage of high frequency somatic embryogenesis (59.01%) was obtained adding 1.0 mg l -1 6-bencylaminopurine in the culture medium. Maturation of somatic embryos was increased using 6.0% sucrose. The greater percentage of somatic embryos germination (76.17%) was reached in the culture medium without growth regulator. A reproducible indirect somatic embryogenesis protocol from callus formation to somatic embryos germination in Swietenia macrophylla King was established.
Authors and Affiliations
Raúl Collado, Raúl Barbón, Daniel Agramonte, Felipe Jiménez-Terry, Martha Pérez, Odalys Gutiérrez
Keywords
Related Articles
- EP ID EP683815
- DOI -
- Views 130
- Downloads 0
In vitro establishment of axillary buds of Bambusa vulgaris var Vittata
Micropropagation is a viable alternative for bamboo propagation due to the potentialities this type of plant has for building, handicrafts confection and soil protection. The technique is an efficient alternative to pla...
Effect of planting distance of in vitro plants on Dioscorea rotundata Point cv. ‘White Guinea’ minituber production in greenhouse
Considering the limited availability of planting material in yam cv. ‘White Guinea’ (Dioscorea rotundata Point), mainly due to the tubers that are consumed are used as seed, have been developed methods for its micropro...
In vitro conservation of sugar cane plants
The development of in vitro culture techniques for plant tissues provides new alternatives for ex situ conservation of sugarcane germplasm. The aim of this paper was to determine the conditions for in vitro conservatio...
Bioactive seaweed extracts as biostimulants of growth and protection of plants
Seaweed extracts are widely used in agriculture as plant growth biostimulants and constitute an ecological alternative to the excessive consumption of synthetic agrochemicals. These bioproducts are complex mixtures of se...
Effect of lead on imbibition, germination, and growth of Phaseolus vulgaris L. and Zea mays L.
Lead is highly reactive and it can be consequently toxic to living cells to both plants and humans. This heavy metal is a source of contamination to the environment and it disrupts natural cycles. The present study was...