In vitro establishment of axillary buds of Bambusa vulgaris var Vittata
Journal Title: Biotecnología Vegetal - Year 2007, Vol 7, Issue 3
Abstract
Micropropagation is a viable alternative for bamboo propagation due to the potentialities this type of plant has for building, handicrafts confection and soil protection. The technique is an efficient alternative to plants propagation with a high genetic value and quality. The current research was developed focused on the in vitro establishing of axillary buds of Bambusa vulgaris var. Vittata. Three concentrations of sodium hypochlorite (1.0, 2.0 and 3.0%) and three different times (10, 15, 20 minutes) were used for disinfection of axillary buds. The highest rates of established buds were achieved using sodium hypochlorite to 2.0% during 20 minutes for disinfection. Between 86% and 100% buds sprouted, and a 93.7% of explants free of visible microbial contaminants were obtained from axillary buds introduced to in vitro conditions.
Authors and Affiliations
Yudith García-Ramírez , Marisol Freire-Seijo , Lillien Fajardo , Marisol Tejeda , Maritza Reyes
Keywords
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