Determination of the minimum inhibitory concentration (MCI) of Hygromycin B for its use as a selection agent in the transformation of Mycosphaerella fijiensis
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 2
Abstract
The Black sigatoka can be considered from the economic point of view the most serious disease in the gender Musa. At present the use of new biotechnological techniques applied to the study of the host-pathogen interation is glimpsed as an important tool in the future improvement of the commercial cultivars of the banana and plantain. In this study, we tested diferents concentration of Hygromycin B against an isolate of M. fijiensis (CCIBP-1) by the agar dilution method. It was proved that Hygromycin B was able to inhibit the growth of the M. fijiensis starting from 0.5 mg.l -1. This value was taken as the minimum inhibitory concentration, that’s why it could be used like selective agent in the transformation studies of Mycosphaerella complex. By another hands it was demostrated the value of the previous protocol of the Minimum Inhibitory concentration for making evaluations of different fungus pathogens against antimicribial substance.
Authors and Affiliations
Mileidy Cruz-Martín, Mayra Acosta-Suárez, Edrey A. Rodríguez, Michel Leiva, Yelenys Alvarado Capó
Keywords
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