Use of Temporary Immersion Systems (RITA) in the propagation of plants via organogenesis in sugar cane and bananas
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 1
Abstract
The present work had as objective to study the possibilities to use the RITA, in the micropropagation plant via axillary buds of sugarcane and of the banana hybrid (Musa sp. AAAA cv. FHIA-01 and AAAB cv. FHIA-18). Two different culture medium i.e. multiplication and thickenment were studied in the multiplication phase of two varieties of sugarcane. It was also studied the number of initial explants to use (two, four and six), using as control flasks with explants liquid and semisolid medium. The number of immersions in 24 hours was analysed and also the time for culture media change was determined testing two periods, 15 days and 30 days respectively. Similar variables were studied for the case of the banana cultivar except for the medium culture. It was obtained like half of more appropriate cultivation for sugarcane that of multiplication with two explants, in the two used varieties, not presenting significant differences the times of change of half made to the 15 and 30 days. For the case of the banana cultivar employment of five explantes for FHIA-01 and three or five explantes for FHIA-18 to begin the multiplication allowed to reach the best results. Regarding the number of immersions it presented bigger superiority the treatment of three immersions during 24 hours, for both banana cultivars.
Authors and Affiliations
Laisyn Posada-Pérez, Rafael Gómez-Kosky, Maritza Reyes, Lizvadis Alvares Díaz
Keywords
Related Articles
- EP ID EP697866
- DOI -
- Views 42
- Downloads 0
Genetic transformation by Agrobacterium tumefaciens in papaya hybrid from in vitro plant shoot-tips
In papaya (Carica papaya L.) viral infections can decrease production by more than 50%. Genetic transformation consists of an alternative means of obtaining new varieties, which are resistant to this pathogen. The main...
Effect of Sodium Chloride on the in vitro development of cultivars 'Gran enano' and 'FHIA 03'
The effect of Sodium Chloride levels on in vitro development of Gran Enano (AAA) and `FHIA 03`(AABB) cultivars was determined. Researches were carried out at Instituto de Investigaciones en Viandas Tropicales (INIVIT)...
Protocol forin vitro propagation of Zingiber officinale Roscoe via organogenesis
Ginger (Zingiber officinale Roscoe) is traditionally propagated by rhizomes and can carrydiseases that decrease production.In vitro propagation allows healthy seed to beobtained, in high volumes and in less time. The obj...
Phytochemical and antibacterial properties of leaf extract of Hamelia patens Jacq.
The firebush (Hamelia patens Jacq.) is a bush widely distributed in tropical areas and used in the traditional medicine for the treatments of wound and inflammatory process. The aim of the present work was to determine...
Genetic stability among in vitro eggplant clones induced by different plant growth regulators
Many factors may influence the genetic stability of cloned in vitro plants, among which are the genotype and the regenerative process induced by plant growth regulators. The resulting somaclonal variations may be usefu...