INDUCTION OF POLYPLOIDS AND ISOLATION OF PLOIDY VARIANTS THROUGH STOMATAL PARAMETERS IN BOUGAINVILLEA (BOUGAINVILLEA SPP)
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 1
Abstract
Even though many varieties available in Bougainvillea, novelty in plant traits is appreciated to meet the demand of landscape industry. In this present investigation, anti-mitotic agents like colchicine and oryzalin were used to induce ploidy variations in two Bougainvillea cultivars of Lalbagh and Mahara. Of the putative polyploids of the cvs. Lalbagh and Mahara, plants with stomatal densities ranging between 13.00 and 22.00, 10.00 and 21.00 stomata mm-2 and stomata size between 78.93 and 159.32, 78.93 and 169.56 µm2 respectively were categorized as diploids whereas those with stomatal densities between 5 and 9 stomata mm-2 and stomatal size between 176.20 and 672.98, 182.42 and 398.09 µm2 respectively were categorized as tetraploids. The proportion of various ploidy levels based on the stomatal traits revealed that 0.4 % colchicine recorded the highest rates of tetraploids (26.67 %) and 100 µM oryzalin recorded the highest rates of mixoploids (27.78 %) in cv. Lalbagh. In case of Mahara, the highest rate of putative tetraploids (16.67 %) was observed in 0.3 % colchicine and 100 µM oryzalin, whereas 0.3 % colchicine recorded 11.11 per cent of mixoploids.
Authors and Affiliations
ANITHA K, JAWAHARLAL M, JOHN JOEL A, SURENDRANATH R
Keywords
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