Influence of chlorpyrifos on some physiological and biochemical indices of Danio rerio
Journal Title: Біологія тварин - Year 2019, Vol 21, Issue 1
Abstract
Physiological and biochemical parameters of Danio rerio fish with toxic injury by chlorpyrifos, one of the most well-known organophosphorus compounds, an active substance of many pesticides were studied. The studies were conducted on adult Danio rerio fishes with an average weight of 0.6 g per 10 individuals per group. To determine the toxicity of chlorpyrifos, the experimental groups of fishes were placed in 5 l aquariums for 96 hours with water in which chlorpyrifos was added in the range of concentrations of 0.375–2 mg/l. Biochemical studies were carried out in blood plasma, which was taken from fish at different concentrations and duration of exposure to the xenobiotic. The parameters of the acute toxicity of chlorpyrifos for Danio rerio were determined. With experiment duration of 96 hours, the maximum concentration of chlorpyrifos, in which the death of D. rerio fish was not fixed, was 0.375 mg/l, the absolute lethal concentration was 2 mg/l, half-lethal concentration (LC50) was 1.23 mg/l. Danio rerio intoxication by selected concentrations and duration of chlorpyrifos was accompanied by a decrease of cholinesterase activity in blood plasma; at the influence of chlorpyrifos at the concentration of 1 mg/l, an increase in the number of hemolysed erythrocytes was observed at 16.5 % (P<0.05), which may indicate a structural damage to the protein-lipid matrix of erythrocytes membranes, while the duration of hemolysis of erythrocytes of experimental fish compared to control did not undergo significant changes. The obtained results prove the efficiency of the use of Danio rerio fish as a biological model for toxicological studies of organophosphorus compounds, in particular chlorpyrifos.
Authors and Affiliations
V. V. Dovhanyuk, V. P. Rosalovsky, Y. T. Salyha
Keywords
Related Articles
- EP ID EP614394
- DOI 10.15407/animbiol21.01.014
- Views 69
- Downloads 0
Blood plasma protein of carp under the influence of sulfanilamide
The protein spectrum of the blood plasma of carp doublets according to the data of PAGE is characterized by the presence of 17 fractions of proteins located in the zones of prealbumins, albumins, transferrins, ceruloplas...
Dynamics of microstructural changes of white rat liver under the influence f e-selen in complex with Closaverm-A
The aim of the study was to examine the histostructural changes of liver at administration of E-selen in complex with Closaverm-A in therapeutic dose during 14 days. To this end, we used 48 white rats at the age of 2 3 m...
Dynamics of phagocytic activity of neutrophils in rats under oxidative stress and the action of liposomal preparation
The article deals with the researches results of the developed complex liposomal preparation influence on dynamics of nonspecific level of natural resistance in rats under modeled oxidative stress caused with the use of...
Histological structure of separate internal organs of piglets at application of probiotic feed additive
The aim of the test was to study the influence of probiotic feed additive in doses recommended by the producer and duration of application on the histological structure of separate internal organs of piglets. The product...
The impact of twinning and stillbirth on reproductive and economic performance in large Hungarian dairy herds
The aim of our study was to quantify the effect of twinning and stillbirth on the main reproductive parameters, and to estimate the resulting economic loss on large-scale commercial dairy farms. The data of 3.660 calvin...