Influence of diversity at the genetic level on the phenotypic diversity parental lines of CMS ogura winter oilseed rape hybrids (Brassica napus L.)
Journal Title: Rośliny Oleiste - Oilseed Crops - Year 2012, Vol 33, Issue 1
Abstract
The selection of components for crossbreeding in order to obtain new improved varieties and hybrids can be carried out both at the plant phenotype and at the genetic level as well.The aim of this study was to examine whether the differentiation of the parental lines of F1 hybrids based on observations of molecular markers would result in their phenotypic variation. The plant materials comprised 18 parental lines of F1 hybrids of winter oilseed rape (Brassica napus L). Nineteen selected phenotypic traits were observed in two localities (Borowo and Zielęcin) in two growing seasons (2002/2003 and 2003/2004). Moreover, the collected material was also characterized at the genetic level by studying the 597 polymorphic molecular markers such as AFLP, RAPD and isozymes. Genetic similarity coefficients were calculated. Cluster analyses were conducted on using the unweighted pair-group method with arithmetic mean and the resulting clusters were shown in a dendrogram. An analysis of variance for the observed traits (with groups designated in the dendrogram as differentiating factor levels) was performed in order to determine whether the variability of individual traits is higher within distinguished groups, or between them. The least significant difference for each investigated trait was calculated at the significance level α = 0.05.The results indicate that the diversity at genetic level was consistent with differences based on phenotypic observations for most of the analyzed traits. Genetic diversity estimated using molecular markers was not consistent with the phenotypic variation for oleic acid (C18:1) content in any of the four examined environments.
Authors and Affiliations
Jan Bocianowski, Alina Liersch, Iwona Bartkowiak-Broda
Keywords
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- EP ID EP59691
- DOI 10.5604/12338273.1058512
- Views 63
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