Influence of sample hemolysis on ELISA test results in blood screening laboratory
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 4
Abstract
Objective To analyze the effect of sample hemolysis on ELISA test results in blood screening laboratory, so as to determine the acceptable tolerance of hemolysis specific to laboratory test items and detection system, and provide reference for the formulation of tolerance standard of sample hemolysis. Methods Negative and weakly positive (S/CO was about 2) samples with different hemolysis degrees were tested by several commonly used domestic reagents for HBsAg, HIV Ag/Ab, anti-HCV and anti-TP, respectively. The effects of various degrees of hemolysis on the test results of negative and weakly positive samples for each item were analyzed. Results 1) Hemolysis had no effect on the test results (reactive/non-reactive) of negative and weakly positive samples for HBsAg, anti-HCV and anti-TP ELISA items; 2) Hemolysis affected the test results (reactive/non-reactive) of negative and weakly positive samples for HIV Ag/Ab ELISA item. A tolerance of Hb 2 g/L was taken as the acceptable hemolysis degree for HIV Ag/Ab ELISA item. Conclusion In this study, the acceptable tolerance of hemolytic samples for corresponding test items and detection system in our laboratory were determined. The influence of hemolysis on ELISA test result is related to the reagent, equipment, environment and other factors, therefore the acceptable tolerance of hemolysis should be determined scientifically and reasonably based on the specific evaluation of each laboratory.
Authors and Affiliations
Qianqian QIN, Hongwei GE, Jing ZHANG, Zifu ZHAO, Chan LENG, Rui WANG
Keywords
Related Articles
- EP ID EP738798
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.04.008
- Views 38
- Downloads 0
Assessment of infection status in treponema pallidum antibody-reactive blood donors
Objective To evaluate the infection status and potential infectivity of Treponema pallidum specific antibody (anti-TP) reactive blood donors, and to provide reference for the key prevention and screening of TP under the...
Adverse reactions to blood transfusion: A retrospective analysis of 814 cases in Qingdao area from 2020 to 2021
Objective To investigate the incidence and composition of adverse reactions to blood transfusion(ARBT) in Qingdao hospitals in recent years. Methods The "Statistical Table of Adverse Reactions to Blood Transfusion in Med...
Different strategies of reducing non-viral nucleic acid of meta-virome in plasma: a comparative study
Objective To explore the influence of common methods of reducing non-viral nucleic acid on the abundance of plasma virus group. Methods Three kinds of library construction, five kinds of centrifugation conditions, two ki...
Diversity of IgG autoantibodies of 4 Chinese intravenous immunoglobulins
Objective To develop methods to display the IgG autoantibody repertoire of intravenous immunoglobulin (IVIG) products, analyze the different types of antibodies and study the diversity of IgG autoantibody in 4 IVIG prepa...
Analysis of laboratory tests and prevention strategies for hemolytic disease of the fetus and newborn caused by anti-M
Objective To analyze the application of serological test results in the diagnosis and treatment of anti-M-induced hemolytic disease of the fetus and newborn(HDFN), and to explore HDFN prevention strategies. Methods The s...