INHIBITION ACTIVITY OF WATER HYACINTH LEAF EXTRACT (EICHHORNIA CRASSIPES) AGAINST AGGREGATIBACTER ACTINOMYCETEMCOMITANS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 6
Abstract
Objective: Water hyacinth (Eichhornia crassipes) contains bioactive compounds that have antibacterial properties. The antibacterial agent is required in the treatment of periodontitis. The aim of this study was to determine the inhibition activity of water hyacinth leaf extract against Aggregatibacter actinomycetemcomitans that was the major cause of aggressive periodontitis. Methods: This research divided into two groups (treatment and control group). The treatment group was conducted using a serial dilution of water hyacinth leaf extract at concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78% in brain heart infusion broth media with A. actinomycetemcomitans suspension. The control group was prepared without water hyacinth leaf extract. All media were incubated at 37°C for 24 h. Inhibition activity was measured by calculates the number of bacterial colonies on Mueller-Hinton agar media. The data were statistical analysis using one-way ANOVA. Results: There were significant differences between the treatment group (concentrations of 100% to 1.56%) and the control group (p < 0.05). Water hyacinth leaf extract at concentrations of 100% to 6.25% showed that there was no growth of A. actinomycetemcomitans. Conclusion: The results suggest that water hyacinth leaf extract has inhibition activity against A. actinomycetemcomitans at a minimal concentration of 1.56%.
Authors and Affiliations
YASINTA IZZAH AFIDATI , IRMA JOSEFINA SAVITRI , AGUNG KRISMARIONO
Keywords
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