Inhibitory and killing activities of black tea (Camellia sinensis) extract against Salmonella enterica serovar Typhi and Vibrio cholerae O1 biotype El Tor serotype Ogawa isolates
Journal Title: Jundishapur Journal of Microbiology - Year 2011, Vol 4, Issue 2
Abstract
Introduction and objectives: Previous studies have shown the potential antibacterial activity of black tea, Camellia sinensis, extract against many pathogenic bacteria including Gram-positives (Listeria monocytogenes and Staphylococcus aureus) and Gram-negatives (Vibrio cholerae and Salmonella enterica serovar Typhi). The aim of the current study was to assess the in vitro antibacterial activity of C. sinensis ethanolic extract (CAS) against multidrug-resistant (MDR) clinical isolates of S. Typhi and V. cholerae O1 biotype El Tor serotype Ogawa (V. cholerae Ogawa ). Materials and methods: The zone diameter of inhibition (ZDI) and minimum inhibitory concentration (MIC) values of CAS for S. Typhi and V. cholerae Ogawa isolates were determined by agar diffusion and agar dilution techniques. Bacterial killing studies were carried out in order to assess the bactericidal activity of CAS against S. Typhi and V. cholerae Ogawa, from Kolkata, India. Results: For the S. Typhi and V. cholerae Ogawa isolates, the ZDIs of CAS ranged 12-17mm and 13-21mm, respectively, and the MICs of the extract were 400-600µg/ml and 200-600µg/ml, respectively. The CAS had bactericidal action at concentrations 512µg/ml and 256µg/ml, respectively for S. Typhi and V. cholerae Ogawa. Conclusions: The black tea (C. sinensis) extract could be useful in combating emerging drug-resistance among enteropathogens including S. Typhi and V. cholerae Ogawa.
Authors and Affiliations
Shyamapada Mandal, Manisha DebMandal, Nishith Kumar Pal, Krishnendu Saha
Keywords
Related Articles
- EP ID EP155191
- DOI -
- Views 130
- Downloads 0
Hemolysin production, salt tolerance, antibacterial resistance, and prevalence of extended spectrum β-lactamases in Proteus bacilli isolated from clinical and environmental sources
Introduction and objective: Proteus bacilli are opportunistic members of Enterobacteriaceae and Proteus mirabilis is among the most common causes of community or hospital acquired urinary tract infections (UTI) in many c...
In vitro antifungal susceptibility of Candida species isolated from oropharyngeal lesions of patients with cancer to some antifungal agents
Introduction and objective: Oropharyngeal candidiasis is a relatively common mycotic infection in cancer patients. In vitro susceptibility of oropharyngeal Candida isolates can be useful in selecting the appropriate trea...
A first case of tinea imbricata from Iran
Tinea imbricata is an uncommon dermatophytosis caused by the anthropophilic dermatophyte Trichophyton concentricum in endemic regions. In the present study, a 10-year-old girl was examined for tinea imbricata. Microscopi...
Detection of Salmonella strain by rapid-cycle multiplex PCR
Introduction and objective: Salmonellosis is responsible for large numbers of infections in both humans and animals. Conventional methods of isolation of Salmonella strains take 4-7 days to complete and are therefore lab...
A case report of tropical rat mite infestation Ornithonyssus bacoti (Dermanyssidae: Acarina) in Ahvaz, SW Iran
Ornithonyssus bacoti (tropical rat mite) is associated with rats throughout the world. Infestation of human follows by rat control or when the rodent is not available. It`s bite is painful, causing intense itching and a...