Insulin-like growth factor - I (IGF-I) complexes in human saliva
Journal Title: Journal of Stomatology (Czasopismo Stomatologiczne) - Year 2017, Vol 70, Issue 6
Abstract
Introduction: At the moment, are detected in the saliva of various biologically active peptides, which include, inter alia, insulin-like growth factors: IGF-I and IGF-II. They circulate in plasma in the form of complexes with binding proteins - IGFBP. Aim of the study: Determination of expression, content and form of IGF-I presented in saliva of healthy persons at different period of life. Material and methods: Investigated material was resting mixed saliva, obtained from 70 healthy volunteers divided into 7 groups, taking into account their age and gender. Western immunoblot and ELISA test was used for the qualitative and quantitative evaluation of complexes of IGF-I. Results: Human saliva contained IGF-I mainly in high molecular mass complexes. The content of free IGF-I was very low and independent of sex and age. Conclusion: Those complexes were formed with its binding proteins, most likely with binding protein - 3 or with binding protein - 5. Western immunoblot in non-reducing conditions makes visible single band with high molecular weight, about 150 kDa, corresponding to IGF/binding proteins/acid labile subunit complex
Authors and Affiliations
Ewelina Dargiewicz, Małgorzata Wolańska
Keywords
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