Interaction of Cholera Toxin B Subunit with Intestinal Epithelial Cells
Journal Title: Journal of Endocrinology and Diabetes - Year 2017, Vol 4, Issue 3
Abstract
We have prepared 125I-labeled cholera toxin B subunit (125I-labeled CT-B, a specific activity of 98 Ci/mmol), and found that its binding to rat IEC-6 intestinal epithelial cells was high-affinity (Kd 3.7 nm). The binding of labeled protein was completely inhibited by unlabeled thymosin-α1 (TM-α1), interferon-α2 (IFN- α2), and the synthetic peptide LKEKK that corresponds to residues 16-20 in TM-α1 and 131-135 in IFN-α2 (Ki 1.5, 1.0 and 2.0 HM, respectively), but was not inhibited by the synthetic peptide KKEKL with inverted amino acid sequence (Ki > 10 mm). Thus, TM-α1, IFN-α2, and the peptide: LKEKK bind with high affinity and specificity to CT-B receptor on riec-6 cells. It was found that CT-B and the peptide: LKEKK at concentrations of 10 - 1000 nm increased in a dose-dependent manner the nitric oxide production and the soluble guanylate cyclase activity in the cells.
Authors and Affiliations
Elena V. Navolotskaya, Vladimir B. Sadovnikov, Valery M. Lipkin, Vladimir P. Zav’yalov
Keywords
Related Articles
- EP ID EP333789
- DOI 10.15226/2374-6890/4/3/00178
- Views 122
- Downloads 0
Global Burden of Diabetes- Rising Expenditures on Healthcare Economy
The dynamics of the diabetes epidemic are changing rapidly. It is a matter of concern on global scale from a public health perspective and also in relation to rising societal costs. Once a disease of the West, type 2 dia...
Prevalence and Social Determinants of Type 2 Diabetes in a Coastal Area of Kerala, India
Introduction: Varying prevalence rates of type 2 diabetes have been observed in different parts of the southern state of Kerala, India which is in an advanced stage of epidemiologic transition. Social patterning is evide...
Effect of Age, Gender and Season on Thyroid Hormones Status in Children of East Delhi- A Hospital Based Study
Background: Thyroid hormones are the most commonly prescribed tests in the clinical set up. Variations are observed in individuals of different age, sex, race and nutritional status. However few studies describe these ch...
Brachial-ankle Pulse Wave Velocity and Renal Function can Predict Coronary Artery Disease in Asymptomatic Japanese Patients with Type 2 Diabetes
In patients with diabetes mellitus, myocardial perfusion defects are often observed in the absence of obstructive Coronary Artery Disease (CAD), thereby presenting a diagnostic problem. Meanwhile, the indications for Mul...
Why OSA may be Regarded as the Real Hidden Killer in Diabetes
Obstructive Sleep Apnea Syndrome (OSAS) is a common and usually overlooked phenomenon causing a deep reduction in oxygen delivery to the body tissues and a serious disruption of the sleep pattern. Furthermore there are s...